安哥拉山羊胚胎的手术回收和移植

对３５只安哥拉山羊超排处理，配种后第３天放置阴道海绵栓，第６日由子宫手术回收胚胎。３１只发情配种的供体，共回收胚胎２０４枚，平均每只供体获可用胚４．３±３．９６枚。其中卵巢有功能黄体无大卵泡的６只供体，回收胚胎５１枚，回收率为８７．１５％±１５．９８％，平均每只获可用胚８．１±２．９６枚。卵巢上有大卵泡、或子宫及输卵管有病变者可使回收率和可用胚胎数严重降低。由于配种后放置了阴道栓，８只有黄体退化倾向的供体，平均每只仍获得可用胚４．６枚。移植桑椹胚和囊胚的５１只受体，３６只妊娠，妊娠率为７０．６％．移植４～１６细胞胚胎的对照组，妊娠率为５１．６％．本研究还证明，移植单胚较移植双胚的胚胎成羔率提高２８％以上

Surgical Collection and Transfer of Embryos in Angora Goat

female Angora goats pretreated with progesterone vaginal sponges for 14 days were superovulated with FSH and 31 goats showing estrous were mated.Vaginal sponges were inserted again on the 3rd day after mating and embryos were collected surgically on the 6th day.204 embryos were recovered with a mean recovery rate of 75.9%, among which 65.7% embryo was transferable.Each donor yielded 4.3 transferable embryo on average.However,51 embryos were collected from 6 donors with 57 functional corpora lutea(CL) and without large folicles.The recovery rate and the number of transferable embryo decreased seriously when there were large follicles on ovaries or pathological changes in uteri.Vaginal sponge inserted after mating prevented in a certain degree embryo degeneration resulted from CL degeneration.Therefore,37 transferable embryos were collected from 8 donors with a tendency of CL degeneration.70.6%(36/51) recipients became pregnant after transfered morulae and blastocysts collected from uteri whereas 51.6% recipients did for the 4-16 cell embryos transfered and collected from oviducts.The survival rate of the embryo was 20% higher in single embryo transfer than that in double embryo transfer.