水稻RACK1基因过表达载体的构建与转化

利用过表达基因技术将从水稻中分离克隆的RACK1基因定向克隆至植物中间表达载体pCAMBIA1301中,构建了RACK1过表达融合基因植物表达载体pCAMBIA1301/R,通过根癌农杆菌EHA105将其导入水稻基因组。对获得的抗性植株的报告基因、基因组PCR及Southern Blotting进行检测分析。结果表明,该过表达基因已整合到水稻基因组中。

Construction and Transformation of RACK1 Gene Over-expression Vector in Rice

The paper aimed at establishing an experimental system for RACK1 gene function study and providing the intermidiate material for further studying the RACK1 gene function in rice.Util-izing over-expression technology,the RACK1 gene cloned from leaves of rice was inserted directly into the plant intermediate expression vector pCAMBIA1301,from which an over-expression fusion gene and a plant expression vector pCAMBIA1301/R were constructed.Genetic transform-ation to rice was mediated by EHA105.Transgenic assays were performed using GUS report,PCR and Southern Blotting.Result showed that the over-expression fusion RACK1 gene had been integrated into rice genome.