| 携带遗传转化质粒的农杆菌在无抗性选择压二次悬浮下的质粒稳定性 |
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| 引用本文： | 师校欣,王英,杜国强,王燕霞,王晓蔓,裴东.携带遗传转化质粒的农杆菌在无抗性选择压二次悬浮下的质粒稳定性[J].分子植物育种,2009,7(5):966-971. |
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| 作者姓名： | 师校欣 王英 杜国强 王燕霞 王晓蔓 裴东 |
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| 作者单位： | 1. 河北农业大学园艺学院,保定,071001
2. 中国林业科学研究院林业研究所,北京,100091 |
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| 基金项目： | 国家863计划项目，河北省自然科学基金项目 |
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| 摘 要： | 本试验对携带不同目的基因和启动子的农杆菌菌株在去掉抗生素条件下进行菌液二次悬浮时的质粒稳定性进行了研究。结果表明：GV3101菌株中的几丁质酶基因质粒与AGL1菌株中的CBF3/rd29a质粒非常稳定,未发生质粒丢失现象;其次是LBA4404菌株中的CpTI质粒,虽发生个别质粒丢失,但与对照相比没有显著差异;携带CBF3/rd29a质粒的EHA105菌株悬浮培养12h后质粒发生明显丢失;含β-1,3葡聚糖酶基因质粒的EHA105菌株、含CBF3/CaMV35S质粒的GV3101菌株悬浮培养10h后质粒发生明显丢失;EHA105菌株CBF1/rd29a质粒和AGL1菌株CBF3/CaMV35S基因质粒最不稳定,分别悬浮培养6h和4h后即发生质粒明显丢失,且随悬浮培养时间的延长,质粒丢失率显著增高。质粒丢失程度与目的基因、启动子和农杆菌菌株三要素中的单一因素没有规律性联系,而是与目的基因、启动子和农杆菌菌株的组合有关;同时,在悬浮时间为0.5～6.0h范围内,质粒丢失主要与菌液浓度有关,而与悬浮时间关系不大;直接吸取一次悬浮液或离心后分别悬浮于细菌培养液和植物培养液等不同的悬浮培养方式下质粒稳定性没有差异。
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| 关 键 词： | 根癌农杆菌 质粒 抗生素 二次悬浮培养 稳定性 |
Genetic Transformation Plasmid Stability of Agrobacterium tumefaciens under the Conditions of the Second Suspend Liquid Culture and without Selective Pressure of Antibiotics |
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| Shi Xiaoxin Wang Ying Du Guoqiang Wang Yanxia Wang Xiaoman Pei Dong College of Horticulture,Agricultural University of Hebei,Baoding, Research Institute of Forestry,Chinese Academy of Forestry,Beijing.Genetic Transformation Plasmid Stability of Agrobacterium tumefaciens under the Conditions of the Second Suspend Liquid Culture and without Selective Pressure of Antibiotics[J].Molecular Plant Breeding,2009,7(5):966-971. |
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| Authors: | Shi Xiaoxin Wang Ying Du Guoqiang Wang Yanxia Wang Xiaoman Pei Dong College of Horticulture Agricultural University of Hebei Baoding Research Institute of Forestry Chinese Academy of Forestry Beijing |
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| Institution: | Shi Xiaoxin 1 Wang Ying 1 Du Guoqiang 1 Wang Yanxia 1 Wang Xiaoman 1 Pei Dong 2 1 College of Horticulture,Agricultural University of Hebei,Baoding,071001,2 Research Institute of Forestry,Chinese Academy of Forestry,Beijing,100091 |
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| Abstract: | In this research genetic transformation plasmid stability of Agrobacterium tumefaciens was studied under the conditions of the second suspend liquid culture and without selective pressure of antibiotics,the tested plasmids harboured with different target genes and their promoters.The results showed that plasmids harboring Chitinase gene in GV3101 strain and plasmids harboring CBF3/rd29a gene in AGL1 strain both had much more stable,which no any plasmids lost during the second suspend liquid culture.Although... |
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| Keywords: | Agrobacterium tumefaciens Plasmid Antibiotic Second suspend incubation Stability |
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