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利用分子标记辅助育种技术选育高抗白叶枯病恢复系
引用本文:邓其明,王世全,郑爱萍,张红宇,李平. 利用分子标记辅助育种技术选育高抗白叶枯病恢复系[J]. 中国水稻科学, 2006, 20(2): 153-158
作者姓名:邓其明  王世全  郑爱萍  张红宇  李平
作者单位:1.四川农业大学 水稻研究所, 四川 成都 611130;2.四川省农业生物工程技术研究中心, 四川 成都 611130; 3四川农业大学 西南作物基因资源与遗传改良教育部重点实验室, 四川 雅安 625014
基金项目:国家科技攻关项目;教育部长江学者奖励计划
摘    要:通过常规回交育种结合分子标记辅助选择技术,已将来自IRBB24的2个抗白叶枯病基因, Xa21和Xa4, 聚合到感病的杂交稻恢复系绵恢725中。通过分子标记检测目标基因和亲本遗传背景差异分析快速获得了4个高抗白叶枯病的恢复系姊妹系R207 1、R207 2、R207 3和R207 4。采用与目标抗性基因相应的菲律宾菌系P1、P6 和7个中国病原型代表菌系(CⅠ~CⅦ)对4个姊妹系及其杂交组合进行田间接种。结果表明,这些姊妹系及所配制的杂交组合抗病性强、抗谱广,其中杂交组合G46A/R207 2具有良好的产量潜力,将R207 2定名为蜀恢207。

关 键 词:白叶枯病  抗性  基因聚合  杂交水稻  恢复系  分子标记辅助选择  育种  
文章编号:1001-7216(2006)02-0153-06
收稿时间:2005-01-07
修稿时间:2005-10-15

Breeding Restorer Lines with High Resistance to Bacterial Blight in Hybrid Rice by Using Molecular Marker-Assisted Selection
DENG Qi-ming,WANG Shi-quan,ZHENG Ai-ping,ZHANG Hong-yu,LI Ping. Breeding Restorer Lines with High Resistance to Bacterial Blight in Hybrid Rice by Using Molecular Marker-Assisted Selection[J]. Chinese Journal of Rice Science, 2006, 20(2): 153-158
Authors:DENG Qi-ming  WANG Shi-quan  ZHENG Ai-ping  ZHANG Hong-yu  LI Ping
Abstract:Two bacterial blight(BB)resistance genes, Xa21 and Xa4,from IRBB24 were introduced into hybrid rice restorer line Mianhui 725, which is highly susceptible to BB by using hybridization and molecular marker-assisted selection technology. Four homologous restorer lines were obtained through molecular marker test for the R target genes and different parental genetic background analysis. Inoculation on the 4 lines and their hybrids were carried out with the specific Xanthomonas oryzae pv.oryzae strains, P1, P6(Philippine races) and seven representative strains of Chinese pathotype, CⅠ-CⅦ.The results indicated that all the 4 lines and their hybrids were highly resistant and showed broad resistance-spectrum to BB. The hybrids of G46A / R207-2 displayed good agronomic characters and high yield potentiality, and R207-2 was named as Shuhui 207.
Keywords:bacterial blight    resistance    gene pyramiding    hybrid rice    restorer line    molecular marker-assisted selection  breeding
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