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| 利用AhMITE1转座子分子标记鉴定花生F1代杂种 | |
| 引用本文： | 王洁,李双铃,王辉,石延茂,任艳,何国浩,袁美.利用AhMITE1转座子分子标记鉴定花生F1代杂种[J].花生学报,2012,41(2):8-12. |
| 作者姓名： | 王洁 李双铃 王辉 石延茂 任艳 何国浩 袁美 |
| 作者单位： | 1. 大连工业大学生物工程学院,辽宁大连116034／山东省花生研究所,山东青岛266100 2. 山东省花生研究所,山东青岛,266100 3. Department of Agricultural Sciences, College of Agricultural, Environmental and Natural Sciences, Tuskegee University, Tuskegee, AL36088, USA 4. 大连工业大学生物工程学院,辽宁大连116034／Department of Agricultural Sciences, College of Agricultural, Environmental and Natural Sciences, Tuskegee University, Tuskegee, AL36088, USA |
| 基金项目： | 山东省博士基金项目，作物种业科技工程项目，留学人员科技活动择优资助项目，国家自然科学基金项目，科技部“863”项目 |
| 摘 要： | 对杂交后代进行杂种的真实性鉴定是杂交育种成功的前提。AhMITE1转座子标记是一类基于PCR的分子标记,其在花生中扩增的产物片段差异大,多态性强,采用琼脂糖凝胶电泳即可区分。本研究用7对多态性转座子引物对6个花生组合的166个F1单株进行了真实性鉴定。根据杂种植株含有父母本带型为依据共鉴定出了77个单株为真杂种,真杂种植株数占植株总数的比率为44.86%;收获时根据田间表型鉴定出了79个单株为真杂种,真杂种比率为47.59%。 |
| 关 键 词： | 花生 杂种鉴定 分子标记 转座子 AhMITE1 PCR |
Identification of Peanut F1 Hybrid Using AhMITE1 Transposable Element Marker |
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| WANG Jie,LI Shuang-ling,WANG Hui,SHI Yan-mao,REN Yan,HE Guo-hao,YUAN Mei.Identification of Peanut F1 Hybrid Using AhMITE1 Transposable Element Marker[J].Journal of Peanut Science,2012,41(2):8-12. | |
| Authors: | WANG Jie LI Shuang-ling WANG Hui SHI Yan-mao REN Yan HE Guo-hao YUAN Mei |
| Institution: | 1 .School of Biological Engineering, Dalian Polytechnic University, Dalian 116034, China; 2 .Shandong Peanut Research Institute, Qingdao 266100, China; 3. Department of Agricultural Sciences College of Agricultural, Environmental and Natural Sciences, Tuskegee University, Tuskegee, AL 36088, USA) |
| Abstract: | Accurate identification of F1 hybrid is critical to hybridization breeding in peanut. MITE transposable marker is a type.of PCR marker with high polymorphism and easy detection by agarose gel electrophoresis. 7 pairs of polymorphic primers were used to amplify 166 F1 individual plants from 6 crosses. Out of those plants, 77 plants were identified as true hybrids, accounting for 44,86%. Based on phenotype and agronomic traits, 79 plants were identified as true hybrids, accounting for 47.59%. |
| Keywords: | Arachis hypogaea L hybrid identification molecular marker transposable element AhMITE1 PCR |
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