|
|||||
|
|
Characterization of and radioimmunoassay for canine thyroglobulin |
|
| Authors: | C P Verschueren P J Selman J J M de Vijlder and J A Mol |
| Institution: | a Department of Clinical Sciences of Companion Animals, University of Utrecht, The Netherlands b Department of Experimental Pediatric Endocrinology, University of Amsterdam, The Netherlands |
| Abstract: | Canine thyroglobulin (cTg) has been isolated and purified. It has similar electrophoretic patterns as Tg from other mammalian species. The main fraction had a MW of 660,000, whereas also fractions of a MW of approximately 1,300,000 (dimer) and 330,000 (subunit) were present. The iodine content was 0.8 to 1.0 % (w/w). cTg did not cross-react with antibodies against human Tg to a degree that would allow the use of a radioimmunoassay for human Tg for the determination of cTg in serum or plasma. Therefore a polyclonal antiserum was raised against cTg and a homologous radioimmunoassay was developed, which was sensitive (0.4 μg/l) and specific (cross-reactivity in cTg assay of human Tg, goat Tg, T4, T3, and DIT < 0.01 %). Plasma Tg levels in normal dogs of both sexes and aged 3–15 years amounted to 192 ± 73 μg/l (mean ± SD, n=30). There was no relation between plasma Tg and T4 levels. |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ | |