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石楠组培快繁技术研究
引用本文:任继文. 石楠组培快繁技术研究[J]. 甘肃林业科技, 2009, 34(2): 15-17
作者姓名:任继文
作者单位:甘肃林业职业技术学院,甘肃,天水,741020
摘    要:对石楠茎段组织培养中外植体的灭菌、褐变预防、增殖培养、根诱导等进行了试验研究。结果表明:外植体诱导再生新梢时,在培养基中添加500mg/L的PVP可有效地控制褐变;以6-BA1.0+IBA0.2为增殖和扩繁培养基效果较好,增殖倍数达5.5;幼茎在1/2MS+IAA(0.1~0.3)mg,L的培养基上生根效果普遍较好,生根率可达90%左右;幼苗经炼苗后盆栽,成活率95%。

关 键 词:石楠  组织培养  快速繁殖

Study on the Technique of Tissue Culture and Rapid Propagation of Photinia serrulata
PEN Ji-wen. Study on the Technique of Tissue Culture and Rapid Propagation of Photinia serrulata[J]. Journal of Gansu Forestry Science and Technology, 2009, 34(2): 15-17
Authors:PEN Ji-wen
Affiliation:REN Ji-wen(Gansu Forestry Technological College,Tianshui Gansu 741020,China)
Abstract:The study was conducted on the experiment of explants sterilization of tissue culture of Photinia serru-lata stem segment,prevention of browning,proliferation culture and root induction and so on.The results indicated: when explants induced newshoot regeneration,turning brown were prevented effectively if adding 500 mg/L PVP in the cultured medium;the effect of cultured medium was better using cultured medium of 6-BA1.0+IBA0.2 proliferation and expanding propagation,proliferation times reached 5.5;the roota...
Keywords:Photinia serrulata  tissue culture  rapid propagation  
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