| 哺乳动物胚胎性别鉴定试剂盒的研究 |
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| 引用本文： | 洪桂云,陈宏权.哺乳动物胚胎性别鉴定试剂盒的研究[J].畜牧与兽医,2006,38(1):19-21. |
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| 作者姓名： | 洪桂云 陈宏权 |
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| 作者单位： | 1. 安徽建筑工业学院环境工程系,安徽,合肥,230022
2. 安徽农业大学动物科技学院,安徽,合肥,230036 |
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| 基金项目： | 安徽省“十五”重大科技专项(01603006) |
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| 摘 要： | 依据牛、山羊、兔、猪等哺乳动物SRY基因高度同源性设计一对22 bp的SRY引物,按照3×2×3因子组合,建立了PCR扩增胚胎DNA最适条件。采用此最适条件扩增了15个羊-兔异种克隆胚胎DNA,结果表明PCR法可以用来鉴别哺乳动物胚胎的性别。采用设计的性别鉴定引物,按照最优PCR扩增胚胎DNA条件配制了PCR性别鉴定试剂盒。
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| 关 键 词： | PCR 克隆胚胎 性别鉴定 试剂盒 |
| 文章编号： | 0529-5130（2006）01-0019-03 |
| 收稿时间： | 2005-06-22 |
| 修稿时间： | 2005-06-22 |
Development of sex identification kit of mammals embryos |
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| HONG Gui-yun,CHEN Hong-quan.Development of sex identification kit of mammals embryos[J].Animal Husbandry & Veterinary Medicine,2006,38(1):19-21. |
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| Authors: | HONG Gui-yun CHEN Hong-quan |
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| Abstract: | A pair of primers with 22 bases in length were designed based on regions of sequence homology among cattle,goats,rabbits and pigs.Optimal embryo DNA PCR conditions for SRY primers were developed using 3×2×3 factorial combination.Using the optimized conditions,fifteen goat-rabbit interspecies clone embryos DNA were amplified.The result showed that PCR could be used to identify the mammal embryo sex.On basis of the optimal embryo DNA PCR conditions for SRY primers,the PCR sex determination kit was developed. |
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| Keywords: | PCR clone embryos sex identification kit |
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