| 农杆菌介导bar基因转化玉米幼胚的研究 |
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| 引用本文： | 邸宏,卢翠华,王振华,刘昭军.农杆菌介导bar基因转化玉米幼胚的研究[J].东北农业大学学报,2008,39(2):150-154. |
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| 作者姓名： | 邸宏 卢翠华 王振华 刘昭军 |
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| 作者单位： | 1. 东北农业大学农学院,哈尔滨,150030
2. 黑龙江省农业科学院生物技术中心,哈尔滨,150086 |
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| 基金项目： | 基金项目：黑龙江省博士后基金项目（LBH-Z06229） |
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| 摘 要： | 通过农杆菌介导法,采用抗除草剂基因(bar)转化3个玉米自交系的幼胚,并对遗传转化影响因素进行优化。结果表明,2,4-D浓度为2 mg·L-1时,胚性愈伤组织诱导率较高;农杆菌最佳的侵染时间为20 min;乙酰丁香酮(AS)明显提高了幼胚的GUS瞬时表达频率,适宜浓度为200μmol·L-1;温和选择方法适于幼胚转化,不定芽诱导培养基、芽伸长培养基和生根培养基中潮霉素的浓度分别为8、6、2 mg.L-1;获得7株PCR检测以及除草剂抗性试验阳性植株。
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| 关 键 词： | 玉米 农杆菌 抗除草剂基因(bar) |
| 文章编号： | 1005-9369（2008）02-0150-05 |
| 收稿时间： | 2006-12-20 |
| 修稿时间： | 2006年12月20 |
Study on transformation of bar gene into maize(Zea mays L.)by Agrobacterium tumefacien mediated |
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| DI Hong,LIU Zhaojun,LU Cuihua,WANG Zhenhua.Study on transformation of bar gene into maize(Zea mays L.)by Agrobacterium tumefacien mediated[J].Journal of Northeast Agricultural University,2008,39(2):150-154. |
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| Authors: | DI Hong LIU Zhaojun LU Cuihua WANG Zhenhua |
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| Abstract: | bar gene was introduced into 3 maize inbred lines by Agrobacterium tumefacien mediated.The results showed that 2 mg·L-1 2,4-D was good to embryogenic callus.The suitable duration of infection was 20 min.The addition of AS(200 μmol·L-1) in infection and co-culture medium enhanced the instant expression frequency of immature embryo.The herbicide concentration should be lower than lethal concentration,8 mg·L-1 was better.Moderate selection fitted for transformation,the best screening mode was to use hygromycin in the concentration of 8,6 and 2 mg·L-1 in induction medium,buds elongation medium and rooting medium respectively.Seven plants showed positive reaction by PCR analysis and were confirmed to resist basta. |
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| Keywords: | maize(Zea mays L ) Agrobacterium tumefacien bar gene |
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