Biofunctionalised polycaprolactone fibrous mat as a transfer tool for cell sheet engineering |
| |
Authors: | Bernadette K. Madathil Sneha Sundaran P. T. V. Kumary Anugya Bhatt P. R. Anil Kumar |
| |
Affiliation: | 1.Division of Tissue Culture, Department of Applied Biology, Biomedical Technology Wing,Sree Chitra Tirunal Institute for Medical Sciences and Technology,Thiruvananthapuram,India;2.Division of Thrombosis Research, Department of Applied Biology, Biomedical Technology Wing,Sree Chitra Tirunal Institute for Medical Sciences and Technology,Thiruvananthapuram,India |
| |
Abstract: | Cell sheet technology is a unique technique in tissue engineering where cell sheets are generated from thermoresponsive substrates, by simple variation of temperature. Though the cell sheets have intact cell-cell and cell-extra cellular matrix junctions, the transfer of large sheets needs a carrier or transfer tool to prevent it from rolling and folding. In this study polycaprolactone electrospun mats with porous fibers (PPCL) functionalized with thrombin is proposed as a cell sheet transfer tool. The biofunctionalised mats were evaluated for cytocompatibility and analyzed for its efficiency to form a clot when exposed to blood plasma. This property was utilized to use the mats for cell sheet engineering. The efficacy of the biofunctionalised mat as a carrier was successfully demonstrated in the transfers of human lung epithelial and mouse fibroblast cell sheets generated from a thermoresponsive N-Isopropylacrylamide-co-Glycidylmethacrylate substrates. The clot acts like a sacrificial adhesive between the cells and the mat making the later to be used as a carrier tool in cell sheet engineering. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|