| 石刁柏花药培养再生单倍体植株和染色体数变异 |
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| 引用本文： | 张磊,刘贵仁,严仁玲,王震星,杨恩芹.石刁柏花药培养再生单倍体植株和染色体数变异[J].天津农学院学报,1995(2). |
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| 作者姓名： | 张磊 刘贵仁 严仁玲 王震星 杨恩芹 |
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| 作者单位： | 天津农学院园艺系!天津300381 |
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| 摘 要： | 石刁柏花药接种在MS 2,4-Dlmg/l 6—BAlmg/1 NAA0.1mg/l的培养基上产生愈伤组织.诱导率为46.15%.愈伤组织转接到MS 6-BA0.5mg/l十IBA0.2mg/l时分化芽.而形成根的培养基为MS NAA0.1mg/l.愈伤组织诱导胚状体的培养基为MS十6-BA0.5mg/l IBA0.5mg/l,胚状体萌发形成小植株.再生植株的倍性变化,单倍体植株占11.90%,二倍体28.57%,四倍体9.52%,各种混倍体50%.
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| 关 键 词： | 石刁柏 小植株 胚状体 染色体倍性 |
Regeneration of Haploid Plantlets From Anthers of Asparagus and Chromosome Numbers Variation |
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| Zhang Lei Liu Guiren Yan Renling Wang Zhenxing Yang Enqin.Regeneration of Haploid Plantlets From Anthers of Asparagus and Chromosome Numbers Variation[J].Journal of Tianjin Agricultural College,1995(2). |
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| Authors: | Zhang Lei Liu Guiren Yan Renling Wang Zhenxing Yang Enqin |
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| Abstract: | Anthers of Asparagus cultured on MS medium containing 1mg/l 2, 4-D 1mg/l 6-BA 0. 1mg/l NAA produced the most callus. The frequency of anther induction reached as high as 46. 15%. Shoot differentiation occurred when callus were transferred to medium containing 0. 5mg/l 6-BA 0. 2mg/l IBA and rooted plantlets were formed on medium containing 0. 1mg/l NAA. Embryoids were induced from callus on basal medium with 0. 5mg/l 6-BA 0. 5mg/lIBA. Germination of plantlets were observed from these embryoids. The regenerated plants varied in ploidy, 11. 90% were haploid, 28. 57% diploid and 9. 53% tetraploid. The others 50% were mixoploid. |
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| Keywords: | Platttlets Embryoids Chromosome ploidy |
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