| 航天紫花苜蓿中黄酮的提取及抑菌活性 |
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| 引用本文： | 董晓宁, 赵海福, 赵强, 移瑞瑞, 靳正娟. 航天紫花苜蓿中黄酮的提取及抑菌活性[J]. 草业科学, 2014, 8(4): 771-775. |
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| 作者姓名： | 董晓宁 赵海福 赵强 移瑞瑞 靳正娟 |
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| 作者单位： | 1.天水师范学院生命科学与化学学院，甘肃天水740001;2.甘肃农业大学动物医学院，甘肃兰州730070;3.1. 天水师范学院生命科学与化学学院，甘肃天水740001;4.2. 中国科学院西北高原生物研究所，青海西宁810001;5.;6.2. 西北师范大学化工学院，甘肃兰州730070 |
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| 摘 要： | 用索氏回流法正交提取航天紫花苜蓿(Medicago sativa)中的黄酮(芦丁)，以510 nm最大吸光度进行可见光谱检测，测定芦丁含量，并对两种供试菌进行体外抑菌活性测定。结果表明，通过单因素试验确定的各因素影响能力大小为超声提取时间料液比乙醇浓度，芦丁含量最高为10.04 mg·g-1;大肠埃希氏杆菌的最小抗菌浓度为0.40 mg·g-1，金黄色葡萄球菌的最小抗菌浓度为0.70 mg·g-1。
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| 关 键 词： | 航天苜蓿 黄酮 抑菌 |
| 收稿时间： | 2013-04-27 |
Study on extraction technology and antibacterial of flavonoids from space breeding alfalfa |
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| Xiao-ning DONG, Hai-fu ZHAO, Qiang ZHAO, Rui-rui YI, Zheng-juan JIN. Study on extraction technology and antibacterial of flavonoids from space breeding alfalfa[J]. Pratacultural Science, 2014, 8(4): 771-775. |
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| Authors: | Xiao-ning DONG Hai-fu ZHAO Qiang ZHAO Rui-rui YI Zheng-juan JIN |
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| Affiliation: | 1.College of Life Science and Chemistry, Tianshui Normal University, Tianshui 741001, China;2.College of Vetetinary Medicine at Gansu Agriculture Univesity, Lanzhou 730070, China;3.1. College of Life Science and Chemistry, Tianshui Normal University, Tianshui 741001, China;4.2. Northwest Plateau Institute of Biology, Chinese Academy of Sciences, Xining 810001, China;5.;6.2. Department of Chemistry, Northwest Normal Uniuersity, Lanzhou 730070, China |
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| Abstract: | Flavonoid was extracted from space breeding alfalfa with Soxhlet extraction with orthogonal design and content was determined with spectrophotometer at 510 nm.Two bacteria were selected for in vitro antibacterial activity test.The results show that each factor with various influence with the following order: Ultrasonic extraction timeLiquid to solid ratioEthanol concentration.The highest content was 10.04 mg·g-1.The MIC of Escherichia coli and Staphylococcus aureus were 0.40 and 0.70 mg·mL-1,respectively. |
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| Keywords: | space breeding alfalfa flavonoids antibacterial |
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