| 一种观察甜菜胚囊的压片法 |
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| 引用本文： | 刘丽萍,李荣田,满玉华.一种观察甜菜胚囊的压片法[J].中国糖料,2006(2):33-34. |
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| 作者姓名： | 刘丽萍 李荣田 满玉华 |
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| 作者单位： | 黑龙江大学,生命科学学院,哈尔滨,150080 |
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| 基金项目： | 国家转基因植物研究和产业化专项基金 |
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| 摘 要： | 胚囊观察是植物胚胎学的主要研究方法之一。我们在学习了酶解法之后,摸索研究了一种观察甜菜胚囊的压片法。其步骤:固定、硼砂洋红整体染色、酸解离、剥离胚珠、透明、压片、观察。观察到了胚囊的一些情况。此法简捷易行,成本低。
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| 关 键 词： | 甜菜 胚囊 压片法 |
| 文章编号： | 1007-2624(2006)02-0033-02 |
| 收稿时间： | 2005-10-13 |
| 修稿时间： | 2005年10月13 |
A Pressure Cover Glass Method of Observation on Embryo Sacs of Sugarbeet |
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| LIU Li-ping,LI Rong-tian,MAN Yu-hua.A Pressure Cover Glass Method of Observation on Embryo Sacs of Sugarbeet[J].Sugar Crops of China,2006(2):33-34. |
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| Authors: | LIU Li-ping LI Rong-tian MAN Yu-hua |
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| Institution: | College of Life Science, Heilongjiang University, Harbin 150080, China |
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| Abstract: | The embryo sacs observation is one of the main study method for embryology. After we learnt enzymatic isolation method of YANG Hong-yuan and ZHOU Chang, we explored to develop a pressure cover glass method of observation on embryo sacs. The procedures are fixation, entire staining, isolation with acid, denudating ovule, transparence, pressuring cover glass and observation. We had observed some circumstances of embryo sacs and macrospore mother cell from sugarbeet, Aztec marigold (Tagetes erecta L.) and Common Petunia(Petunia hybrida Vilm.). The method is simple, direct, easily handled and lower cost. |
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| Keywords: | Sugarbeet Embryo sacs Pressure cover glass method |
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