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| 栗属中国特有种—板栗、茅栗、锥栗RAPD分析 | |
| 引用本文： | 杨剑,唐旭蔚,涂炳坤,谢谱清.栗属中国特有种—板栗、茅栗、锥栗RAPD分析[J].果树学报,2004,21(3):275-277. |
| 作者姓名： | 杨剑 唐旭蔚 涂炳坤 谢谱清 |
| 作者单位： | 1. 深圳职业技术学院生物应用工程系,广东,深圳,518055 2. 华中农业大学林学系,湖北,武汉,430070 3. 武汉市林业果树科学研究所,湖北,武汉,430062 |
| 摘 要： | 对中国栗属特有种板栗(CastaneamollissimaBl.)、茅栗(C.seguiniiDode.)、锥栗(C.henryiRehd.&Wils.)10个样品进行了RAPD分析,24个引物共获得158条谱带,其中102条为多态性谱态。利用UPGMA法构建遗传关系聚类图,以相似性系数0.62为阈值,板栗与茅栗为一个聚类组,锥栗为另一个聚类组,表明板栗与茅栗的亲缘关系较近,两者与锥栗的亲缘关系相对较远。 |
| 关 键 词： | 板栗 茅栗 锥栗 RAPD 聚类分析 |
| 文章编号： | 1009-9980(2004)03-275-03 |
| 修稿时间： | 2003年7月31日 |
RAPD Analysis of Three Chinese Endemic Castanea Species |
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| Yang Jian,Tang Xuwei,Tu Bingkun,and Xie Puqing.RAPD Analysis of Three Chinese Endemic Castanea Species[J].Journal of Fruit Science,2004,21(3):275-277. | |
| Authors: | Yang Jian Tang Xuwei Tu Bingkun and Xie Puqing |
| Institution: | Yang Jian1,Tang Xuwei1,Tu Bingkun2,and Xie Puqing3 |
| Abstract: | RAPD analysis of 10 samples of three Chinese endemic Castanea species, Castanea mollissima Bl., C. sequinii Dode. and C. henryi (Skan) Rehd. et Wils. was carried out by using 38 arbitrary 10 bp primers. Totally 158 bands were obtained, of which 102 bands were polymorphic. The genetic tree was constructed according to the method of UPGMA. C. mollissima and C. sequinii belonged to one cluster group while C. henryi belonged to another group at the similarity of 0.62 in the tree; it indicated that the genetic relationship between C. mollissima and C. sequinii was close whereas the relationship of these two species were distant to another species, C. henryi. |
| Keywords: | Castanea mollissima C sequiini C henryi RAPD Cluster analysis |
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