| 牛磺酸对卵形鲳鲹肠道微生物及免疫功能的影响 |
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| 引用本文： | 马启伟,郭梁,刘波,刘宝锁,朱克诚,郭华阳,张楠,杨静文,张殿昌. 牛磺酸对卵形鲳鲹肠道微生物及免疫功能的影响[J]. 南方水产科学, 2021, 17(2): 87-96 |
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| 作者姓名： | 马启伟 郭梁 刘波 刘宝锁 朱克诚 郭华阳 张楠 杨静文 张殿昌 |
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| 作者单位： | 上海海洋大学水产与生命学院,上海 201306;中国水产科学研究院南海水产研究所/农业农村部南海渔业资源开发利用重点实验室,广东 广州 510300;中国水产科学研究院南海水产研究所/农业农村部南海渔业资源开发利用重点实验室,广东 广州 510300;广东省海洋生物种业工程技术研究中心,广东 广州 510300 |
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| 基金项目： | 现代农业产业技术体系建设专项资金(CARS-47-G07);中国水产科学研究院中央级公益性科研院所基本科研业务费专项资金资助(2020TD29);广东省现代农业产业技术体系海水鱼产业创新项目(2019KJ143);国家自然科学基金项目(U20A2064)。 |
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| 摘 要： | 为了探讨牛磺酸对卵形鲳鲹(Trachinotus ovatus)肠道微生物菌群结构和免疫功能的影响,试验以鱼粉、发酵豆粕和玉米蛋白粉为基础蛋白源配制了牛磺酸质量分数分别为1.3 g·kg-1(T0)、4.4 g·kg-1(T1)、7.4 g·kg-1(T2)、10.5 g·kg-1(T3)、12.7 g·kg-1(T4...
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| 关 键 词： | 卵形鲳鲹 牛磺酸 肠道微生物 免疫功能 |
Effect of taurine on intestinal microbes and immune function in golden pompano(Trachinotus ovatus) |
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| MA Qiwei,GUO Liang,LIU Bo,LIU Baosuo,ZHU Kecheng,GUO Huayang,ZHANG Nan,YANG Jingwen,ZHANG Dianchang. Effect of taurine on intestinal microbes and immune function in golden pompano(Trachinotus ovatus)[J]. South China Fisheries Science, 2021, 17(2): 87-96 |
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| Authors: | MA Qiwei GUO Liang LIU Bo LIU Baosuo ZHU Kecheng GUO Huayang ZHANG Nan YANG Jingwen ZHANG Dianchang |
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| Affiliation: | (College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China;South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences/Key Laboratory of South China Sea Fishery Resources Exploitation&Utilization,Ministry of Agriculture and Rural Affairs,Guangzhou 510300,China;Guangdong Provincial Engineer Technology Research Center of Marine Biological Seed Industry,Guangzhou 510300,China) |
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| Abstract: | In order to investigate the effects of taurine on the intestinal microflora structure and immune function of Trachinotus ovatus,we used fish meal,fermented soybean meal and corn gluten meal as the basic protein sources to prepare the nitrogen and fat feed with taurine contents of 1.3 g·kg-1(T0),4.4 g·kg-1(T1),7.4 g·kg-1(T2),10.5 g·kg-1(T3),12.7 g·kg-1(T4),respectively.Seven hundred and fifty individuals of T.ovatus with an average body mass of(80.0±0.5)g were randomly devided into five groups with three replicates of fifty fish,and the experiment lasted 56 d.Sequencing results show that a total of 87707 sequences had been obtained in the intestine of T.ovatus.The number of OTUs that can be annotated to the database was 5130(95.32%).At phylum level,Proteobacteria,Tenericutes and Spirochaetes were the predominant phyla.Alpha diversity and beta diversity analyses show that the richness and diversity of intestinal microbial in T2 group were the lowest and the species composition was significantly different between T1 and T4 groups(P<0.05).The serum lysozyme activity,C4,and Ig increased significantly with the addition of exogenous taurine(P<0.05).The expression of ToTLR-1,ToTLR-2,ToTNF-αand ToIL-1βin each group decreased significantly(P<0.05).The expression of ToNFkB P65 of T1,T2 and T4 groups was significantly lower than that of the control group(P<0.05),while the expression of ToIL-10 of T3 and T4 groups was significantly higher than that of control group(P<0.05).The results indicate that exogenous taurine has a significant impact on intestinal microflora and immune function. |
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| Keywords: | Trachinotus ovatus Taurine Intestinal microbes Immune function |
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