琅琊鸡及其配套系蛋壳质量、钙代谢生化指标和钙结合蛋白CaBP-D28k mRNA表达的比较

[目的]探讨影响琅琊鸡及其配套系蛋壳质量和钙代谢的差异因素,为琅琊鸡品种资源保护及开发提供理论依据.[方法]选取240日龄琅琊鸡及其浅麻羽色、深麻羽色配套系各180只,各品系随机分为6个重复,每个重复30只,在相同饲养条件下饲养至300日龄时各重复随机收集鸡蛋30枚,各重复随机选取6只,翅下静脉采集血样、屠宰后收集左腿...

Comparison Analysis on Eggshell Quality,Biochemical Index of Calcium Metabolism and Calcium Binding Protein CaBP-D28k mRNA Expression Between Langya Chicken and Its Synthetic Lines

【Objective】The aim of this research was to provide a theoretical basis for the conservation of Langya Chicken breed resources and the development of its synthetic lines based on the comparative study on eggshell quality and calcium metabolism of Langya Chicken and its two synthetic lines. 【Method】Each of one hundred and eighty 240-day-old birds of Langya Chicken and its two synthetic lines (Light linen and Dark linen synthetic lines), were randomly divided into 6 repeats, each with 30 replicates. When the chicken were raised to 300 days of age under the same breeding conditions, thirty eggs and six birds were randomly collected from each repeat, blood samples were taken from the veins under the wings, and tissue samples from the left leg tibia and duodenum, egg shell glands, and kidneys were collected after slaughter. Calcium- and phosphorus-related indicators and the expression levels of calcium binding protein CaBP-D28k mRNA were detected. 【Result】The results showed that the egg weight of the light linen synthetic line was significantly lower than that of the dark linen synthetic line (P<0.05), and the eggshell weight was lower than other lines (P>0.05). In addition, the eggshell thickness and eggshell ratio were significantly higher than those of the linen line (P<0.05), the strength of eggshell was significantly higher than that of other lines (P<0.05). The calcium content of egg yolk, eggshell, and tibia in light linen synthetic line was significantly higher than that in dark linen synthetic line (P<0.05). The content of phosphorus in the tibia was significantly higher than that of other lines (P<0.05), and the ash content in eggshells and tibia was the highest in the light linen synthetic line, followed by the Langya Chicken and the dark linen synthetic line (P<0.05). The body weight and tibia length of light linen synthetic line was significantly lower than those of dark linen synthetic line (P<0.05). The contents of plasma calcium and calcitonin in the light linen synthetic line were significantly lower than those in other lines, while the plasma phosphorus, alkaline phosphatase, and parathyroid hormone contents in the light linen synthetic line were significantly higher than those in other lines, and the differences between the lines were significant (P<0.05); the calcium binding protein content of the light linen synthetic line was significantly lower than that of the dark linen synthetic line (P<0.05). While the osteocalcin of the synthetic lines was significantly higher than that of the Langya Chicken (P<0.05). The expression level of calcium binding protein CaBP-D28k mRNA in duodenum of light linen synthetic line was significantly higher than that of other lines (P<0.05), and the expression level in eggshell gland was significantly higher than that of dark linen synthetic line (P<0.05). The expression level of calcium binding protein in kidney was significantly higher than that of Langya Chicken (P<0.05). 【Conclusion】The above results showed that the plasma phosphorus, alkaline phosphatase, parathyroid hormone, osteocalcin and other related active substances and calcium-binding protein CaBP-D28k mRNA expression level in duodenum, kidney, and shell glands of light linen synthetic lines was all higher than other lines during the 240-300 day-old laying period. which could promote the absorption of calcium and phosphorus in the upper small intestine and the release and transport of bone calcium, affect the deposition of minerals in egg yolk and eggshell, and improve the quality of eggshell and tibia.