| 应用RT-PCR技术检测马铃薯A病毒 |
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| 引用本文： | 耿宏伟,白艳菊,范国权,高艳玲,张威,申宇,马纪,郭怡璠. 应用RT-PCR技术检测马铃薯A病毒[J]. 中国马铃薯, 2009, 23(6): 329-332 |
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| 作者姓名： | 耿宏伟 白艳菊 范国权 高艳玲 张威 申宇 马纪 郭怡璠 |
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| 作者单位： | 1. 黑龙江省农业科学院植物脱毒苗木研究所,黑龙江,哈尔滨,150086
2. 黑龙江省农业科学院作物育种研究所,黑龙江,哈尔滨,150086 |
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| 基金项目： | 马铃薯产业创新体系项目 |
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| 摘 要： | 参考GenBank中马铃薯A病毒(potato virus A,PVA)的保守序列,利用Primer6.0引物设计软件设计并合成了一对特异性引物PVAF、PVAR,以此引物利用RT-PCR方法对PVA保守序列基因进行了特异性扩增。结果表明:引物PVAF、PVAR能从已知的感染PVA病毒的植株中扩增出834bp的cDNA特异性片段;该RT-PCR的检测灵敏度为1pg的病毒核酸,特异性强,重复性好,可用于PVA病毒的快速检测。
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| 关 键 词： | RT-PCR 检测 马铃薯A病毒 |
Detection of Potato Virus A by RT-PCR |
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| Geng Hongwei,Bai Yanju,Fan Guoquan,Gao Yanling,Zhang Wei,Shen Yu,Ma Ji,Guo Yifan. Detection of Potato Virus A by RT-PCR[J]. Chinese Potato, 2009, 23(6): 329-332 |
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| Authors: | Geng Hongwei Bai Yanju Fan Guoquan Gao Yanling Zhang Wei Shen Yu Ma Ji Guo Yifan |
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| Abstract: | A pair of primers PVAF and PVAR were designed according to the segmental nucleotide sequence of conservative gene of potato virus A(PVA) in GenBank to amplify the gene from the healthy plants and the plants which were infected with PVA,potato virus Y(PVY),potato virus X(PVX) and potato virus S(PVS),respectively.Only one PVA specific 834 bp cDNA product was amplified from the plants which were infected with PVA,but not from the others.The detectable viral dsRNA amount of this RT -PCR was 1 pg.This RT -PCR has the good sensitivity and specialty.These results indicate that this RT-PCR could be used for rapid detection of the PVA disease. |
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| Keywords: | RT-PCR |
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