| 伪狂犬病病毒上海株的gI基因的克隆和序列分析 |
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| 引用本文： | 姜焱,侯玉峰,陈德胜,黄伟坚,范伟兴,陈溥言.伪狂犬病病毒上海株的gI基因的克隆和序列分析[J].动物医学进展,2002,23(2):63-65. |
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| 作者姓名： | 姜焱 侯玉峰 陈德胜 黄伟坚 范伟兴 陈溥言 |
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| 作者单位： | 1. 南京农业大学农业部动物疫病诊断与免疫重点实验室,江苏,南京,210095
2. 南京出入境检验检疫局,江苏,南京,210001 |
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| 摘 要： | 参考Genebank发表的伪狂犬病病毒（Pseudorabies Virus,PRV)的gI糖蛋白基因序列，自行设计合成一对引物，对PRV上海株（PRV－SH）进行PCR扩增，产物经琼脂糖电泳分析，呈现一条约960bp的条带，将其克隆入pGEM-T-easy载体中，并进行序列测定，与PRV Rice株gI基因比较发现，核苷酸的同源性为94．7％，氨基酸的同源性为91．3％，证实为gI基因。
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| 关 键 词： | 伪狂犬病病毒 gI基因 序列分析 上海株 基因克隆 |
| 文章编号： | 1007-5038(2002)02-0063-03 |
| 修稿时间： | 2001年8月27日 |
Cloning and Sequencing Analysis of gI Gene of Pseudorabies Virus |
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| JIANG Yan ,HOU Yu feng ,CHEN De shen,HUANG Wei jian ,FAN Wei xing ,CHEN Pu yan.Cloning and Sequencing Analysis of gI Gene of Pseudorabies Virus[J].Progress In Veterinary Medicine,2002,23(2):63-65. |
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| Authors: | JIANG Yan HOU Yu feng CHEN De shen HUANG Wei jian FAN Wei xing CHEN Pu yan |
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| Institution: | JIANG Yan 1,HOU Yu feng 2,CHEN De shen,HUANG Wei jian 1,FAN Wei xing 1,CHEN Pu yan 1 |
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| Abstract: | According to the Genebank open sequence,a pairs of primer was designed in order to amplify gI gene of pseudorabies virus Shanghai strain.The gI gene was obtained by polymerase chain reaction(PCR) ,subsequently cloned into pGEM T vector.The nucleotide and amino acid sequences of SH strain gI gene were compared with the corresponding sequences of Rice strain,the nucleotide and amino acid homology of SH strain with Rice being 94.7% and 91.3% respectively revealed that It is gI gene. |
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| Keywords: | pseudorabies virus gI gene sequencing analysis |
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