水稻卷叶突变新基因的遗传分析和初步定位

利用60Co-γ射线辐照水稻优良恢复系南花6号，获得一个水稻新型卷叶突变材料，整个生育期叶片表现为向内卷曲。经遗传分析表明，该突变性状受一对隐性基因控制。利用突变体南花6号／籼稻品种DularF2君羊体中的141个卷叶单株进行基因定位，在双亲、卷叶和正常叶的DNA池中筛选N2个多态性标记RM285和RM342，并确定该基因位于水稻第9染色体长臂上，与前人报道的r19（f）相距54．7cM，是一个未曾报道的基因，暂时命名为r113（t）。利用SSR标记将该基因定位于第9染色体RM285和RM342之间，遗传距离分别为6．74cM和11．35cM。类似于r113（t）卷叶突变体表型未见报道，该研究结果对揭示卷叶机理及在株型改良应用中具有重要意义。

Genetic analysis and gene mapping of a new rolled leaf mutant in rice (Oryza sativa L.)

In the present study, a new rolled leaf mutant was obtained from rice restoring line Nanhua 6 when treated with 6Co-γ ray. The mutant showed a rolled-leaf phenotype throughout the development stage. The F1, TC1, BC1 and F2 populations, derived from crosses between mutant Nanhua 6 （P1）, cuhivar wild type Nanhua 6 （P2） and Dular （P3） were used for genetic analysis. It was found that the mutant trait of rolled leaf was controlled by a pair of recessive gene. To determine the location of the gene, 141 mutant-type F2 （P1/P3） individuals derived from the cross of mutant Nanhua 6/Dular, were used and the bulked segregant analysis was carried out using DNA pools of mutant and wild-type. The screening with two polymorphic marker, viz, RM285 and RM342 showed that the gene was located on the long arm of chromosome 9. The distance of the gene to reported gene r19 （t） was 54.7 cM, and it has not been reported elsewhere, therefore, temporarily named as r113（t）. By further gene mapping of RM285 and RM342, the gene was found to be located between RM285 and RM342 of the chromosome 9 with 6.74 and 11.35 cM genetic distances. Currently, the rolled-leaf genotype similar to rll3（t） has never been identified near the region. The results provide a basis for r113 （t） gene cloning and its functional analysis, and to study the mechanism related to rolled leaf and the improvement of plant-type.