巴氏芽孢八叠球菌诱变选育脲酶高产菌株

脲酶催化尿素的水解生成形成铵根离子和碳酸根离子，具有广泛的应用价值。以巴氏芽孢八叠球菌ATCC11859为出发菌株, 通过亚硝基胍诱变，得到高产脲酶突变株。其中菌株B\|20的脲酶活性最高，为28.4 mmol/min，较出发菌株提高了207%。对该菌株接种量、氮源种类培养条件进行优化，结果表明接种量为10%，且采用大豆粉为氮源时，脲酶活性最高为32.9 mmol/min。对脲酶高产菌株的选育表明该方法是一种行之有效的方法。

Breeding High\|yield Urease\|producing Sporosarcina pasteurii Strain by NTG Mutation

Urease catalyzed hydrolysis of urea formed ammonium ions and carbonate ions. It has extensive application value. The strain of S. pasteurii ATCC11859 was screened through NTG (N\|methyl\|N″\|nitroso\|N\|nitrosoguanidine) mutation and a high yielding urease mutant was obtained. Among which strain B\|20 gave the highest urease activity of 28.37 mmol/min. This was increased 207% of the original strain. The strain inoculation amount and culture conditions of nitrogen source type were optimized. The results showed that the inoculation quantity was 10%, and using soybean meal as nitrogen source could achieve maximum urease activity as high as 32.9 mmol/min. This indicated that the technology is effective method for breeding high\|yield urease strain.