| 怀山药叶片脱分化过程中细胞早期变化的研究 |
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| 引用本文： | 陈明霞,李明军,郭君丽,张峰,张嘉宝. 怀山药叶片脱分化过程中细胞早期变化的研究[J]. 吉林农业大学学报, 2003, 25(5): 517-519 |
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| 作者姓名： | 陈明霞 李明军 郭君丽 张峰 张嘉宝 |
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| 作者单位： | 河南师范大学生命科学学院,河南,新乡,453002 |
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| 基金项目： | 河南省自然科学基金(98401202)，重点科技攻关项目(0123030900) |
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| 摘 要： | 对怀山药叶片脱分化过程中细胞早期变化进行了研究。结果表明：叶片愈伤组织起源于叶肉细胞；细胞脱分化的特点是细胞质变浓，核相对变大，核仁明显；叶肉细胞脱分化后，其分裂方式为无丝分裂。
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| 关 键 词： | 怀山药 叶片 脱分化过程 无丝分裂 愈伤组织 叶肉细胞 |
| 文章编号： | 1000-5684(2003)05-0517-03 |
| 修稿时间： | 2003-01-13 |
A Study on Early Changes of Cotyledon Cells of Dioscorea opposita Thunb in the Process of Dedifferentiation |
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| CHEN Ming-xia,LI Ming-jun,GUO Jun-li,ZHANG Feng,ZHANG Jia-bao. A Study on Early Changes of Cotyledon Cells of Dioscorea opposita Thunb in the Process of Dedifferentiation[J]. Journal of Jilin Agricultural University, 2003, 25(5): 517-519 |
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| Authors: | CHEN Ming-xia LI Ming-jun GUO Jun-li ZHANG Feng ZHANG Jia-bao |
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| Abstract: | The early changes of cotyledon cells cultured in leaf dedifferentiation of Dioscorea opposita Thunb were studied. The results showed that leaf explant calli were originated from succulent leaf cells. The dynamics in the process of leaf dedifferentiation were reflected in the following ways: cytoplast was concentrated, nuclei were enlarged, and nucleolus became more apparent. Amitosis was the main way of cell division at the stage of cell dedifferentiation. |
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| Keywords: | Dioscorea opposita Thunb leaf dedifferentiation callus amitosis |
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