栽培绿豆V2709抗豆象特性遗传及基因初步定位

 【目的】绿豆[Vigna radiata（L.）Wilczek]是豇豆属亚洲亚属（Ceratotropis）中的一个栽培豆种，豆象是危害绿豆的重要害虫，利用分子标记对抗豆象基因进行初步定位，为该材料在绿豆育种中的利用和抗豆象基因的分离奠定基础。【方法】利用原产于印度的抗豆象栽培绿豆V2709与农艺性状优良的感豆象推广品种中绿1号（VC1973A）配制杂交组合，对杂种F2、BC1F1和F3进行遗传分析；采用BSA法，以上述F2代为定位群体，利用抗、感亲本和抗、感池筛选63个RAPD引物和113对SSR/STS引物。【结果】遗传分析结果表明该抗豆象特性由一对显性基因控制；引物筛选结果表明2个引物能在抗、感池间扩增出多态性条带，用Mapmaker EXP3.0b软件作图，将该抗豆象基因初步定位在一个RAPD标记和一个STS标记之间，遗传距离分别为11.0 cM和5.8 cM，并暂定名为Br2。【结论】本研究定位的基因标记可作为绿豆抗豆象育种分子标记辅助选择的一种工具。

Heredity Analysis and Gene Mapping of Bruchid Resistance of a Mungbean Cultivar V2709

By using V2709, a mungbean cultivar with bruchid resistance from India, and VC1973A, a bruchid susceptible variety form AVRDC as parents, The segregation of bruchid resistance in the F2 and BC1F1 and F3 populations showed that the bruchid resistance of V2709 is controlled by one dominant gene. The local linkage map of one dominant gene harbored in V2709 was constructed with F2 population and three kinds of molecular marker techniques. The results showed that the genetic distances from the target gene to two markers, RAPD and STS, were 11.0cM and 5.8cM, respectively.