江苏省葡萄炭疽病菌群体对4种杀菌剂的敏感性

为明确江苏省葡萄炭疽病菌Colletotrichum gloeosporioides群体对苯醚甲环唑、吡唑醚菌酯、咯菌腈和戊唑醇4种杀菌剂的敏感性和抗性水平，本研究采用菌丝生长速率法测定了来自江苏省7个地区的68株葡萄炭疽病菌对上述4种药剂的敏感性，建立敏感基线，并分析菌株抗性水平和抗性频率。结果表明：苯醚甲环唑、吡唑醚菌酯、咯菌腈和戊唑醇对供试68株葡萄炭疽病菌的EC₅₀范围分别为0.03～1.20、0.05～3.48、0.14～1.20 mg/L和0.50～12.30 mg/L;对苯醚甲环唑、吡唑醚菌酯和咯菌腈的敏感基线分别为(0.53±0.032)(1.00±0.15) mg/L和(0.38±0.024) mg/L。68株菌株对戊唑醇的EC₅₀平均值为3.61 mg/L,因敏感性频率分布不符合建立敏感性基线要求，故未建立敏感性基线。供试菌株对苯醚甲环唑均表现为敏感，对吡唑醚菌酯和咯菌腈呈现一定的低抗水平，抗性频率分别为4.41%和3.03%;对戊唑醇呈低抗和中抗水平，抗性频率分别为16.18%和5.88%。该研究为防控江苏省葡萄炭疽病提...

Sensitivity of Colletotrichum gloeosporioides from grapevine to four fungicides in Jiangsu province

To evaluate the sensitivity and resistance level of Colletotrichum gloeosporioides from Jiangsu province to difenoconazole, pyraclostrobin, fludioxonil and tebuconazole, a total of 100 samples of the infected fruits of grape were collected form seven different regions in Jiangsu province, after isolation and purification, 68 strains were obtained. The sensitivity of the 68 isolates to the four fungicides was determined using mycelial growth rate method. The results showed that the EC50 values of difenoconazole, pyraclostrobin, fludioxonil and tebuconazole to the 68 strains ranged from 0.03 to 1.20 mg/L, 0.05 to 3.48 mg/L, 0.14 to 1.20 mg/L and 0.50 to 12.3 mg/L, respectively. The sensitivity baseline of C. gloeosporioides to difenoconazole, pyraclostrobin and fludioxonil was (0.53±0.032) mg/L, (1.00±0.15) mg/L and (0.38±0.024) mg/L, respectively. The average EC50 value of tebuconazole was 3.61 mg/L, but the sensitivity baseline of C. gloeosporioides to tebuconazole was not established, because of frequency distribution of EC50 values of 68 strains did not meet the requirements for establishing the sensitivity baseline. The tested strains were all sensitive to difenoconazole, low-resistant to pyraclostrobin and fludioxonil with resistant rates of 4.41% and 3.03%, respectively, and low-resistant and medium-resistant to tebuconazole with resistant rates of 16.18% and 5.88%, respectively. This study would provide a reference for reasonable use of fungicides and theoretical basis for scientific prevention and control of grape ripe rot in Jiangsu.