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Suppressive activity of some glucosinolate enzyme degradation products on Pythium irregulare and Rhizoctonia solani in sterile soil
Authors:Luisa M Manici  Luca Lazzeri  Gianluca Baruzzi  Onofrio Leoni  Stefania Galletti  Sandro Palmieri
Abstract:Glucosinolate degradation products (GLDPs), mainly isothiocyanates, obtained by myrosinase‐catalysed hydrolysis of glucosinolates (GLs), are an important group of natural bioactive substances. The fungitoxic activity of four GLDPs, chosen on the basis of their side‐chain structures, was assayed against Pythium irregulare and Rhizoctonia solani. The effectiveness of the degradation products (DPs) of sinigrin (alkenyl GL), epi‐progoitrin (hydroxy‐alkenyl GL), glucoiberin and glucoerucin (thiofunctionalised GLs) in inhibiting P irregulare oospore germination and R solani soil colonisation were tested in a closed system, using an artificially infected soil. The fungitoxic activity of these GLDPs varied according to their side‐chain structure. As in previous in vitro studies, the thiofunctionalised GLDPs were found the most effective, producing complete inhibition of P irregulare oospore germination (0.01 µmole g?1 soil) and R solani soil colonisation (0.5 µmole g?1 soil), but even sinigrin DP showed a fungitoxic activity higher than that of epi‐progoitrin DP. © 2000 Society of Chemical Industry
Keywords:biofumigation  isothiocyanates  myrosinase
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