地黄品种遗传多样性RAPD分析

用CTAB法提取10个地黄品种幼叶的基因组DNA，用紫外分析法和琼脂糖凝胶电泳方法检测基因组DNA的纯度、大小和浓度。结果表明，从80条RAPD引物中分别筛选出适合于地黄RAPD标记分析的引物17条。17条RAPD引物对10个地黄品种（系）扩增出177条带，多态条带比率（PPB）为61．58％，平均多样性指数（I）为0．3135，遗传相似系数（GS）在0．63～0．93，平均GS为0．7545。RAPD标记的聚类分析结果，将10个地黄品种分为2类：第1类含有6个品种，包括组培85—5、大田85—5、组培9302、金白地黄、金状元和大田9302；第2类含有4个品种，包括北京1号、大红袍、地黄9104和野生地黄。

Analysis of Genetic Diversity of Rehmannia glutinosa Based on RAPD Markers

The DNAs were extracted from 10 cultivars(lines) of Rehmannia glutinosa Libosch by means of CTAB method to establish an efficient RAPD-based genetic diversity detection system.The genomic DNA,from a young regenerated plant of Rehmannia glutinosa Libosch f.hueichingensis(Chaoet schih) Hsiao,was used as a template to optimize RAPD-PCR amplification conditions for Rehmannia glutinosa Libosch.The results were as follows: 17 ones from 80 RAPD primers tested were selected for the RAPD analyses of Rehmannia glutinosa Libosch,177 polymorphic bands were amplified by 17 RAPD primers and average proportion of polymorphic loci was 61.58%.The shannon'-s index was 0.3135.Genetic similarity value was from 0.63 to 0.93 and the average was(0.7545).10 Rehmannia cultivars(lines) could be divided into two groups,one contained 6 cultivars(lines) such as Zupei 85-5,Datian 85-5,Zupei 9302,Datian9302,Jinzhuangyuan and Jinbai,the other was composed of 4 cultivars(lines) such as Beijing No.1,Dahongpao,Dihuang9104 and wild dihuang.The results of principal component analysis were very consistent to that by the RAPD markers.