乙酰水杨酸对盐胁迫下番茄蔗糖代谢关键酶活性与基因表达的影响

探讨乙酰水杨酸对盐胁迫条件下苗期番茄体内蔗糖代谢相关酶活性与基因表达的影响,为水杨酸类物质调控设施番茄的耐盐性提供新证据。以栽培番茄“辽园多丽”为材料,用0.05%乙酰水杨酸(ASA),0.16M NaCl,0.16M NaCl 0.05%ASA进行处理,测定并分析了蔗糖代谢相关酶(转化酶、蔗糖合成酶、蔗糖磷酸合成酶)的活性变化,以及转化酶mRNA水平的表达变化。结果表明,乙酰水杨酸在一定程度上可以减弱盐胁迫番茄幼苗生长的抑制。同时乙酰水杨酸可以维持或提高短期(1～3d)盐胁迫条件下番茄幼苗体内转化酶的活性,并使相应的可溶性酸性转化酶mRNA水平很高,而处理后期(5～7d)转化酶活性及可溶性酸性转化酶mRNA水平都下降,但是乙酰水杨酸对盐胁迫条件下番茄幼苗体内蔗糖合成酶、蔗糖磷酸合成酶的影响不大。乙酰水杨酸可通过调节蔗糖代谢相关酶基因的表达引起相应酶活性的变化。

Effects of Acetylic Salicylic Acid on Key Enzyme Activities and Gene Expression of Sucrose Metabolism of Tomato under Salt Stress

The objective of this study was to investigate the effects of acetylsalicylic acid on activity of sucrose-metabolizing enzyme in tomato seedlings under salt stress and to provide a new evidence for tolerating salt stress of cultivated tomato.'Liaoyuanduoli'(Lycopersicon esculentum) was treated with 0.05% acetylic salicylic acid(ASA),0.16 M NaCl and 0.05% ASA 0.16 M NaCl.The related enzymes,activities of sucrose metabolism including invertase,sucrose synthase and sucrose phosphate synthase and the expression of soluble acid invertase mRNA were determined.The results indicated that ASA could reduce the growing restraint of tomato seedlings induced by high salt stress to a certian extent.ASA can maintain or enhance the invertase activities of tomato seedlings in a short period of time(1~3 days),and elevate the expression of soluble acid invertase mRNA,moreover,the invertase activities and the expression of soluble acid invertase mRNA were decreased in later treatment(5~7days).But the effects of ASA treatment on sucrose synthase and sucrose phosphate synthase activities were weak under the condition of salt stress.It showed that ASA could regulate the activities of sucrose-metabolizing enzymes by controlling its gene expression under salt stress