In vitro steroidogenesis by gonads of the Russian sturgeon, Acipenser gueldenstaedti Brandt |
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Authors: | O. Bukovskaya J.G.D. Lambert D.E. Kime |
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Affiliation: | (1) Physiological Institute, St. Petersburg University, University emb., 7/9, St. Petersburg, 199034, Russia;(2) Department of Experimental Zoology, Research Group Comparative Endocrinology, University of Utrecht, Padualaan 8, 3584 Utrecht, The Netherlands;(3) Department of Animal and Plant Sciences, The University of Sheffield, Sheffield, S10 2TN, UK |
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Abstract: | Ovaries and testes from the Russian sturgeon at different stages of sexual maturity were incubated with ;sup3;H-androstenedione or 3H-pregnenolone. The major metabolites of androstenedione in both sexes were testosterone and 5- and 5ß-androstanediols, but no evidence was found for the gonadal production of 11-oxygenated androgens. Pregnenolone was converted to 17-hydroxyprogesterone, androstenedione and testosterone together with reduced metabolites. 11-Oxygenated androgens were found in female serum by gas chromatography-mass spectrometry (GC-MS), in serum of both sexes by radioimmunoassay (RIA), and was detectable by RIA in interrenal but not gonadal tissue. The results suggest that sturgeon may differ from teleosts in that 11-oxygenation may take place in extragonadal tissues. |
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Keywords: | Sturgeon Acipenser gueldenstaedti steroids 11-ketotestosterone ovary testis |
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