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Isolation and characterization of gut bacteria of fruit fly, Bactrocera tau (Walker)
Authors:Chandra S. Prabhakar  Pankaj Sood  Sarbjit S. Kanwar  Prem N. Sharma  Ajay Kumar  Pawan K. Mehta
Affiliation:1. Department of Entomology, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur, Kangra, Himachal Pradesh, 176 062, India
4. International Crops Research Institute for the Semi Arid Tropics, Patancheru, Andhra Pradesh, 502 324, India
5. Crop Research Sub Station, CSK Himachal Pradesh Krishi Vishvavidyalaya, Sundernagar, Mandi, Himachal Pradesh, 175 019, India
2. Department of Microbiology, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur, Kangra, Himachal Pradesh, 176 062, India
3. Molecular Plant Pathology Laboratory, Department of Plant Pathology, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur, Kangra, Himachal Pradesh, 176 062, India
Abstract:Gut bacteria of fruit fly, Bactrocera tau (Walker) (Diptera: Tephritidae), were isolated and the isolates attractive to B. tau adults were characterized using morphological, biochemical and 16S rRNA analyses to determine their taxonomic position. Based upon morphological, biochemical and 16S rRNA sequences (on the basis of closest match), five gut bacterial species of B. tau were characterized as Delftia acidovorans, Pseudomonas putida, Flavobacterium sp., Defluvibacter sp. and Ochrobactrum sp., of which four bacterial isolates, viz., Delftia acidovorans, Flavobacterium sp., Defluvibacter sp. and Ochrobactrum sp. are new records from guts of the fruit fly species.
Keywords:
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