坦布苏病毒囊膜蛋白诱导的小鼠免疫应答

为了研究坦布苏病毒囊膜蛋白(E蛋白)的免疫原性,利用大肠杆菌表达系统高效表达坦布苏病毒囊膜蛋白(E蛋白)并对其诱导小鼠免疫应答进行了研究。结果表明,E蛋白免疫BALB/c小鼠后,免疫组小鼠血清中抗体效价均可达1∶51 200以上;同时,E蛋白可显著诱导血清中细胞因子IL-2、TNF-α和IFN-γ的表达,免疫组小鼠血清中IL-2、TNF-α的含量均极显著高于对照组(P0.01),IFN-γ含量则显著高于对照组(P0.05);此外,与对照组相比,E蛋白还可极显著刺激小鼠脾脏淋巴细胞的增殖,上述结果表明,E蛋白免疫后,小鼠机体可产生显著的体液免疫和细胞免疫反应。攻毒保护性试验结果显示,攻毒后,经荧光定量RT-PCR检测,E蛋白免疫组小鼠脑、肝脏、脾脏、卵巢、肾脏中坦布苏病毒载量均显著低于对照组,表明免疫E蛋白可有效抑制坦布苏病毒在小鼠各个组织中的增殖。结果表明,大肠杆菌表达的坦布苏病毒E蛋白可作为坦布苏病毒亚单位疫苗的候选抗原,为进一步预防和控制坦布苏病毒病奠定理论基础。

Envelope Protein of Goose Tembusu virus Induces Immune Response in Mice

In order to research the immunogenicity of Goose tembusu virus(GTV) envelope protein (E protein),GTV E protein was expressed in Escherichia coli, and administered to BALB/c mice via subcutaneous injection to study the immune responses.After immunization by recombinant E protein, the titres of neutralizing antibody in immunized BALB/c mice were all above 1∶51 200.Cytokine analysis of the serum of mice immunized with recombinant E protein revealed antigen-specific IL-2, TNF-α and IFN-γ responses.IL-2 and TNF-α in serum from immunized mice were extremely significantly higher than control mice (P< 0.01) and IFN-γ was significantly higher than that of control mice (P<0.05).Additionally, the proliferation of lymphocytes from spleen of mice immunized with recombinant E protein was stimulated.The results indicated that recombinant GTV E protein could effectively stimulate humoral and lymphocyte immune responses in mice.Moreover, when mice were challenged with GTV, by using Real-time RT-PCR, the loads of virus in tissues from mice immunized with recombinant E protein were significantly decreased, revealing that recombinant E protein contributed to protection against GTV challenge.This study demonstrated that the E.coli-expressed recombinant GTV E protein could serve as a potential subunit vaccine candidate against GTV and provided a theoretical basis for the prevention and control of Tembusu virus.