| 拟南芥磷酸肌醇特异性磷脂酶C(AtPLC2)的亚细胞定位分析 |
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| 引用本文： | 赵亚婷,韩玉霞,邢红侠,李玉琦,庞茜,邢继红,董金皋. 拟南芥磷酸肌醇特异性磷脂酶C(AtPLC2)的亚细胞定位分析[J]. 河北农业大学学报, 2017, 40(3). DOI: 10.13320/j.cnki.jauh.2017.0055 |
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| 作者姓名： | 赵亚婷 韩玉霞 邢红侠 李玉琦 庞茜 邢继红 董金皋 |
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| 作者单位： | 1. 河北农业大学,真菌毒素与植物分子病理学实验室,河北保定071000;2. 张家口东方中学,河北张家口,075000 |
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| 基金项目： | 河北省自然科学基金项目 |
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| 摘 要： | 为明确拟南芥磷酸肌醇特异性磷脂酶C(AtPLC2)的亚细胞定位情况,本研究构建了AtPLC2基因融合GFP标签的表达载体35S:PLC2-GFP,并通过农杆菌介导的遗传转化方法,将重组质粒转化本氏烟草叶片,转基因烟草叶片细胞利用激光扫描共聚焦显微镜观察,绿色荧光蛋白荧光信号与细胞质膜重合,表明拟南芥AtPLC2基因定位于细胞质膜上,研究结果为明确拟南芥AtPLC2基因的功能奠定了良好的基础。
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| 关 键 词： | 拟南芥 AtPLC2 绿色荧光蛋白 亚细胞定位 |
Subcellular localization of phosphoinositide-specific phospholipase C (AtPLC2) gene in Arabidopsis thaliana |
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| ZHAO Ya-ting,HAN Yu-xia,XING Hong-xia,LI Yu-qi,PANG Xi,XING Ji-hong,DONG Jin-gao. Subcellular localization of phosphoinositide-specific phospholipase C (AtPLC2) gene in Arabidopsis thaliana[J]. Journal of Agricultural University of Hebei, 2017, 40(3). DOI: 10.13320/j.cnki.jauh.2017.0055 |
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| Authors: | ZHAO Ya-ting HAN Yu-xia XING Hong-xia LI Yu-qi PANG Xi XING Ji-hong DONG Jin-gao |
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| Abstract: | To clarify the subcellular localization of AtPLC2 gene in Arabidopsis thaliana,the AtPLC2 gene was cloned and fused with GFP in frame to construct a binary vector 35S:PLC2-GFP.Then,the binary vector was transformed into Nicotiana benthamiana by Agrobacterium tumefaciens-mediated method.Using confocal laser scanning microscopy,green fluorescent signals were observed on plasma membrane of the transformed Nicotiana benthamiana cells.These results indicated that the AtPLC2 gene of Arabidopsis thaliana was localized on plasma membrane. |
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| Keywords: | Arabidopsis thaliana AtPLC2 GFP subcellular localization |
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