响应面法优化超声波辅助提取白头翁总皂苷

采用响应面法优化了超声波法辅助提取白头翁总皂苷(pulsatilla saponin)的条件。以白头翁皂苷B4提取率为评价指标,在单因素试验的基础上,利用Box-Behnken响应面分析法,确定最佳提取条件。结果表明,超声波辅助提取白头翁总皂苷的最佳工艺参数为超声功率240 W,乙醇体积分数60%,超声时间30 min。在此条件下白头翁皂苷提取得率为5.35%。响应面法优化得到的提取工艺稳定合理,准确可靠,是提取白头翁皂苷的可行方法。

The Optimization of the Ultrasonic-assisted Extraction of Total Pulsatilla Saponin by Response Surface Methodology

The optimization of the ultrasonic-assisted extraction of total pulsatilla saponin was studied by using the response surface methodology. The extraction rate of pulsatilla saponin B4 was the evaluation index. On the basis of single factor exper-iment, the optimum extraction condition was determined by using Box-Behnken response surface analysis method. The results showed that ultrasonic-assisted extraction and the optimum technological parameters of total saponins in Pulsatilla root were ultrasonic power of 240 W,60% ethanol, ultrasonic time of 30 min. Under these conditions the extraction rate of pulsatilla saponin was 5.35%. Response surface optimization of the extraction process was stable and reasonable, accurate and reliable, which was a feasible method to extract of pulsatilla saponin.