| 利用抗、染根肿病F_2群体构建大白菜AFLP遗传连锁图谱 |
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| 引用本文： | 王淼,王剑,李宏博,林荣杓,朴钟云.利用抗、染根肿病F_2群体构建大白菜AFLP遗传连锁图谱[J].华北农学报,2009,24(2). |
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| 作者姓名： | 王淼 王剑 李宏博 林荣杓 朴钟云 |
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| 作者单位： | 1. 沈阳农业大学,园艺学院,辽宁,沈阳,110161
2. 忠南大学,农业生命科学学院,园艺系,韩国,大田,305764 |
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| 基金项目： | 国家自然科学基金，国家高技术研究发展计划(863计划) |
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| 摘 要： | 以易感染根肿病的大白菜(Brassica campestris ssp.pekinensis)自交系94SK和抗根肿病的CR Shinki DH系为亲本,通过杂交获得F1,并选择一株F1植株进行自交,构建了F2作图群体.利用扩增出与根肿病抗性基因CRb连锁标记的17对AFLP引物组合Pst+GNN/Mse+CNN构建大白菜遗传连锁图谱.这些AFLP引物组合共扩增出322个亲本间表现出多态性的AFLP标记.应用其中6个与CRb基因紧密连锁的AFLP标记转化成的SCAR标记和清晰可见的大于100 bp的211个AFLP标记构建了一张包含179个标记位点、10个连锁群、覆盖长度为576 cM,平均图距3.3 cM的遗传图谱.抗根肿病基因CRb定位在第一条连锁群9 cM的范围内.研究表明,利用Pst/Mse Ⅰ和EcoR Ⅰ/Mse Ⅰ引物组合以及其他分子标记类型构建遗传连锁图谱,并有效减少遗传图谱中聚集和间隙现象的产生提供了一定的科学依据.同时,该图谱的构建对在基因组范围内通过分子标记辅助选择选育大白菜根肿病抗性品种奠定了基础.
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| 关 键 词： | 大白菜 遗传连锁图谱 根肿病 |
Constructed an AFLP Genetic Linkage Map of Chinese Cabbage with Resistant and Susceptible F_2 Lines |
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| WANG Miao,WANG Jian,LI Hong-bo,LIN Yong-pyo,PIAO Zhong-yun.Constructed an AFLP Genetic Linkage Map of Chinese Cabbage with Resistant and Susceptible F_2 Lines[J].Acta Agriculturae Boreali-Sinica,2009,24(2). |
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| Authors: | WANG Miao WANG Jian LI Hong-bo LIN Yong-pyo PIAO Zhong-yun |
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| Institution: | 1.College of Horticulture;Shenyang Agricultural University;Shenyang 110161;China;2.Department of Horticulture;College of Agriculture and Life Science;Chungnam National University;Daejeon 305764;Korea |
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| Abstract: | Clubroot disease,caused by Plasmodiophora brassicase War.is soil-born,obligate plant pathogen that causes the development of galls on root tissue in Crucifer.The clubroot is one of the most damaging diseases of brassica crops and is the most serious disease of cruciferous crops in the temperate zone.An F2 mapping population was developed from a cross between Chinese cabbage inbred line of 94 SK,a line susceptible to clubroot disease,and CR Shinki DH line,a line resistance to clubroot disease.Seventeen Pst+G... |
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| Keywords: | AFLP |
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