| 不同MS和B 9浓度对马铃薯脱毒试管苗生长的研究 |
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| 引用本文： | 崔翠,王季春,何凤发,周清元,刘希忠,李文斌.不同MS和B 9浓度对马铃薯脱毒试管苗生长的研究[J].西南农业大学学报,2001,23(5):414-417,421. |
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| 作者姓名： | 崔翠 王季春 何凤发 周清元 刘希忠 李文斌 |
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| 作者单位： | [1]西南农业大学农学系，重庆400716 [2]重庆市种子公司，重庆400000 |
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| 摘 要： | 选用米拉、凉薯97、金冠3个品种和MS液体培养基（全量、半量）、B9含量（5mg/L,15mg/L,25mg/L）进行3因素完全随机试验，调查各处理后脱毒试管苗的苗重、株高、茎节、根重和根数。其结果显示：1/2MS培养基和B9浓度为5mg/L时对脱毒试管苗的苗重和株高伸长有促进作用，但在试管苗茎节数的增加上因品种不同选用的MS培养基成分含量及B9浓度有差异。
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| 关 键 词： | 马铃薯 MS培养基 B9 组织培养 试管苗 脱毒 |
| 文章编号： | 1000-2642(2001)05-0414-04 |
INFLUENCES OF THE COMPOSITION OF MS MEDIUM AND B 9 CONCENTRATION ON THE GROWTH OF VIRUS- FREE POTATO PLATLETS IN IN VITRO CULTURE |
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| CUI Cui ,WANG Ji-chun ,HE Feng-fa ,ZHOU Qing-yuan ,LIU Xi-zhong ,LI Wen-bin.INFLUENCES OF THE COMPOSITION OF MS MEDIUM AND B 9 CONCENTRATION ON THE GROWTH OF VIRUS- FREE POTATO PLATLETS IN IN VITRO CULTURE[J].Journal of Southwest Agricultural University,2001,23(5):414-417,421. |
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| Authors: | CUI Cui WANG Ji-chun HE Feng-fa ZHOU Qing-yuan LIU Xi-zhong LI Wen-bin |
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| Institution: | CUI Cui 1,WANG Ji-chun 1,HE Feng-fa 1,ZHOU Qing-yuan 1,LIU Xi-zhong 2,LI Wen-bin 1 |
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| Abstract: | A complete random design was used in a three-factor experiment where potato cultivars Mira, Liangshu 97 and Jinguan were cultured on MS or 1/2 MS media containing B9 at 5, 15 and 25 mg/L. Plant weight, plant height, stem node number, root weight and root number of the virus-free plantlets were recorded. 1/2 MS medium with a B9 concentration of 5 mg/L was most favorable for weight gain and plant elongation of the plantlets for all three cultivars while the optimum composition of the MS medium and B9 concentration for node increase varied with cultivar. |
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| Keywords: | potato MS medium B 9 tissue culture tube plantlet |
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