嘧啶醇在马铃薯试管苗长期保存中的作用

为研究植物生长抑制剂嘧啶醇在马铃薯种质资源试管苗长期保存中的作用,本文对添加嘧啶醇影响试管苗长期保存及其后期存活情况进行了分析。结果表明,17℃保存条件下,添加≥15μmo·lL-1嘧啶醇均可显著降低试管苗的株高。恢复生长研究证明,17℃保存条件下添加≥20μmo·lL-1嘧啶醇可以显著提高试管苗的存活率,保存12个月的试管苗可全部存活,保存18个月后Solanum chacosense和CE76品系仍有70%以上的试管苗可以存活。

Efficacy of Ancymidol for Long-term Storage of Potato Plantlets in vitro

Ancymidol,an inhibitor of plant growth,was investigated in this study as a medium supplement for long-term storage of potato plantlets in vitro.The results showed when more than 15 μmol·L-1 ancymidol was added to the medium,the growth of plantlets could be slowed down and the preservation was prolonged in vitro at 17℃.After 12 months conserved in ancymidol media(≥20 μmol·L-1) at 17℃,all potato plantlets could survive.A total of 70% potato plantlets of Solanum chacosense and CE76 could also survive after 18 months conserved under the same condition.