| 4个桉树无性系愈伤诱导和分化 |
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| 引用本文： | 裘珍飞,曾炳山,李湘阳,刘英,查志刚. 4个桉树无性系愈伤诱导和分化[J]. 林业科学研究, 2012, 25(4): 531-534 |
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| 作者姓名： | 裘珍飞 曾炳山 李湘阳 刘英 查志刚 |
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| 作者单位： | 1. 中国林业科学研究院热带林业研究所,广东 广州,510520
2. 安徽黄山市森林病虫防治检疫站,安徽 黄山,245000 |
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| 基金项目： | "863"项目"白桦、桉树等分子育种与品种创制"(2011AA100202)研究内容 |
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| 摘 要： | 桉树(Eucalyptus L’Hérit.)是世界三大树种之一,随着生物技术的发展,桉树转基因研究正以前所未有的速度发展,而不同的种、无性系胚性愈伤诱导的难易和植株再生能力的强弱直接影响转基因工程实施的进程。尾巨桉(E.urophylla S.T.Blake×E.grandis W.Hill ex Maiden)无性系DH3229、巨尾桉
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| 关 键 词： | 桉树 愈伤诱导 再生率 |
| 收稿时间： | 2011-06-16 |
Induction and Differentiation of Four Eucalyptus Clones in vitro Culture |
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| QIU Zhen-fei,ZENG Bing-shan,LI Xiang-yang,LIU Ying and ZHA Zhi-gang. Induction and Differentiation of Four Eucalyptus Clones in vitro Culture[J]. Forest Research, 2012, 25(4): 531-534 |
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| Authors: | QIU Zhen-fei ZENG Bing-shan LI Xiang-yang LIU Ying ZHA Zhi-gang |
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| Affiliation: | Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou 510520, Guangdong, China;Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou 510520, Guangdong, China;Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou 510520, Guangdong, China;Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou 510520, Guangdong, China;Huangshan Forest Pest Control Quarantine Station, Huangshan 245000, Anhui, China |
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| Abstract: | The in vitro plantlet leaves and stems of 4 elite Eucalyptus clones commonly cultivated in production were used as explants to study the callus induction and differentiation under different callus induction time, in order to establish an effective callus regeneration system. The results showed: The clones Eg5 and DH201-2 leaf callus inducted for 45 d were suitable for differentiation of adventitious buds, with the highest regeneration rate of 70.0% and 63.3% respectively. 20 d and 15 d were the best induction times for leaf callus inducting and stem callus inducting of clone GL9, with the regeneration rates of 66.7% and 63.3% respectively. The best regeneration rate of DH3229 was 16.7% from stem callus inducted for 30 d. There were significant differences in regeneration rate among the 4 clones. Clones Eg5, DH201-2, and GL9 were easy to regenerate compared with DH3229. No obvious difference was found in regeneration rate of leaf callus and stem callus from same clone. |
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| Keywords: | Eucalyptus callus induction regeneration rate |
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