| 利用RAPD技术快速鉴定番茄杂种纯度 |
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| 引用本文： | 栾雨时,苏乔,李海涛,安利佳,何孟元.利用RAPD技术快速鉴定番茄杂种纯度[J].园艺学报,1998(3). |
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| 作者姓名： | 栾雨时 苏乔 李海涛 安利佳 何孟元 |
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| 作者单位： | 东北师范大学遗传与细胞研究所，大连理工大学生化工程研究所，辽宁省农业科学院园艺研究所 |
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| 摘 要： | 从两个番茄杂种Ｌ４０２、Ｌ４０４及它们的亲本共６份材料的叶片中提取ＤＮＡ，使用国产ＰＣＲ仪，建立了适合番茄ＲＡＰＤ分析的ＰＣＲ条件，进一步在１００个随机引物中找到了５个可用于鉴定这两个杂种纯度的引物，其中ＯＰＫ１４引物可同时用来鉴定两个杂种。利用该法鉴定杂种纯度不仅简单、迅速、可靠，而且因ＤＮＡ的用量少，不影响被检植株的后期生长。
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| 关 键 词： | 番茄 RAPD 杂种鉴定 |
Purity Determination of Tomato Hybrid by RAPD Technique |
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| Luan Yushi ,Su Qiao ,Li Haitao ,An Lijia ,and He Mengyuan.Purity Determination of Tomato Hybrid by RAPD Technique[J].Acta Horticulturae Sinica,1998(3). |
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| Authors: | Luan Yushi Su Qiao Li Haitao An Lijia and He Mengyuan |
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| Institution: | Luan Yushi 1,Su Qiao 2,Li Haitao 3,An Lijia 2,and He Mengyuan 1 |
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| Abstract: | Genomic DNAs were extracted from young leaves of two tomato hybrids and their pa rents. PCR conditions suitable for random amplified polymorphic DNA(RAPD)analysis of tomato were studied.Of 100 arbitrary primers used in the experiment,5 primers amplified polymorphic bands between the Paris of parents. The two hybrids could be distinguished from each other using the primer OPK14. |
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| Keywords: | Tomato RAPD Determination of hybrid purity |
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