| 牙鲆迟钝爱德华氏菌血清型及荧光抗体检验 |
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| 引用本文： | 陈翠珍,房海,张晓君,巩元芳,葛慕湘. 牙鲆迟钝爱德华氏菌血清型及荧光抗体检验[J]. 水产科学, 2005, 24(1): 6-8 |
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| 作者姓名： | 陈翠珍 房海 张晓君 巩元芳 葛慕湘 |
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| 作者单位： | 河北科技师范学院,动物科学系,河北,昌黎,066600;河北科技师范学院,动物科学系,河北,昌黎,066600;河北科技师范学院,动物科学系,河北,昌黎,066600;河北科技师范学院,动物科学系,河北,昌黎,066600;河北科技师范学院,动物科学系,河北,昌黎,066600 |
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| 基金项目： | 河北省科技厅科技攻关项目(02220502D) |
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| 摘 要： | 以迟钝爱德华氏菌的代表菌株(HC010907-1)为免疫原,制备免疫血清,对分离于牙鲆鱼的130株迟钝爱德华氏菌进行了血清型检定,结果表明:供试的130株迟钝爱德华氏菌均为同种血清型;同时以此免疫血清为第一抗体,以标准羊抗兔IgG荧光抗体为第二抗体,进行了荧光抗体技术检验迟钝爱德华氏菌的可行性试验,表明亦具有较强的特异性。
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| 关 键 词： | 牙鲆 迟钝爱德华氏菌 血清型 荧光抗体技术 |
| 文章编号： | 1003-1111(2005)01-0006-03 |
| 修稿时间： | 2004-04-21 |
Serotype and Fluorescent Antibody of Edwardsiella tarda Isolated from Flounder(Paralichthys olivaceus) |
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| CHEN Cui-zhen,FANG Hai,ZHANG Xiao-jun,GONG Yuan-fang,GE Mu-xiang. Serotype and Fluorescent Antibody of Edwardsiella tarda Isolated from Flounder(Paralichthys olivaceus)[J]. Fisheries Science, 2005, 24(1): 6-8 |
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| Authors: | CHEN Cui-zhen FANG Hai ZHANG Xiao-jun GONG Yuan-fang GE Mu-xiang |
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| Abstract: | The serological identification of 130 strains Edwardsiella tarda isolated from flounder was conducted using the rabbit antiserum against the selected representative strain (HC010907-1). The results showed that the test 130 strains E.tarda belong to the same serotype. The raised rabbit antiserum was used as the first antibody and goat anti rabbit IgG-FITC was used as the second antibody. The feasible test of fluorescent antibody showed the specificity in examination of E .tarda . |
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| Keywords: | Paralichthys olivaceus Edwardsiella tarda serotype fluorescent antibody technique |
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