碳源对StSte12基因调控玉米大斑病菌生长和分生孢子发育的影响

以葡萄糖、蔗糖、乳糖和可溶性淀粉为碳源分别培养玉米大斑病菌野生型和St Ste12基因RNAi阳性转化子,研究碳源对St Ste12基因调控玉米大斑病菌菌丝生长和分生孢子发育的影响,为阐明St Ste12基因的功能和病菌的致病机制奠定基础。结果表明,St Ste12基因对玉米大斑病菌菌丝生长和分生孢子发育均有重要的调控作用,St Ste12基因的表达量下降后,菌丝的生长势变弱,颜色变浅,生长速度显著下降,产孢量显著降低。4种供试碳源中,可溶性淀粉对St Ste12基因调控菌丝生长和发育的影响最大,蔗糖最小;乳糖对St Ste12基因调控分生孢子发育没有显著影响,而其他3种碳源的影响均达到显著水平。

Effects of Carbon Sources on Regulation of StSte12 to Mycelial Growth and Conidial Development of Setosphaeria turcica

The wild type isolate and StSte12 RNAi positive transformants were cultured on media with glu-cose,lactose,sugar or soluble starch as carbon source,to analyze the effects of carbon sources on regula-tion of StSte12 to mycelial growth and conidial development of Setosphaeria turcica,in order to clarify the function of StSte12 and mechanisms of fungal pathogenicity. The results demonstrated that StSte12 paid a key role in mycelial growth and conidial development of S. turcica. With the decrease of gene expression of StSte12, the mycelial growth ability was weakened, hypha color was lightened, mycelial growth speed was significantly attenuated and the conidial number was significantly decreased. The regulation of StSte12 to mycelial growth was significantly affected by carbon sources, among which the effect of soluble starch was the strongest and the sugar was the weakest. Except lactose,the other three carbon sources had significant-ly effects on regulation of StSte12 to conidial development of S. turcica.