| 海南长春花黄化病植原体的DAPI荧光显微检测 |
| |
| 引用本文： | 陶明福,车海彦,罗大全.海南长春花黄化病植原体的DAPI荧光显微检测[J].热带作物学报,2010,34(2):17-20. |
| |
| 作者姓名： | 陶明福 车海彦 罗大全 |
| |
| 作者单位： | 1 中国热带农业科学院环境与植物保护研究所2 海南省热带农业有害生物检测监控重点实验室3 海南大学环境与植物保护学院;1 中国热带农业科学院环境与植物保护研究所2 海南省热带农业有害生物检测监控重点实验室;1 中国热带农业科学院环境与植物保护研究所2 海南省热带农业有害生物检测监控重点实验室3 海南大学环境与植物保护学院 |
| |
| 基金项目： | 基金项目：国家自然科学基金资助项目(No.30660103)；中央科研院所基本科研业务费专项(No.2009hzs1J012)；国家支 撑计划课题(No.2007BAD48B06)。 |
| |
| 摘 要： | 对感染黄化病的海南长春花植株，应用DAPI荧光显微技术对其嫩茎和叶柄进行了切片染色观察，结果表明：在感病植株的韧皮部筛管分子中存在大量特异性的植原体DNA荧光光点，而在健康植株的筛管分子则未发现有特异性的植原体荧光。另对其茎和叶柄中的植原体含量进行比较，结果显示茎中的含量要比叶柄中稍高。
|
| 关 键 词： | 长春花黄化病 植原体 DAPI检测 |
Detection of Phytoplasma of Hainan Periwinkle Yellows Disease With DAPI Fluorescence Microscopy |
| |
| TAO Mingfu,CHE Haiyan and LUO Daquan.Detection of Phytoplasma of Hainan Periwinkle Yellows Disease With DAPI Fluorescence Microscopy[J].Chinese Journal of Tropical Crops,2010,34(2):17-20. |
| |
| Authors: | TAO Mingfu CHE Haiyan and LUO Daquan |
| |
| Institution: | 1 Environment and Plant Protection Institute, CATAS2 Key Laboratory for Baleful Biology Detection and Monitor of Tropical Agriculture of Hainan Province3 College of Environment and Plant Protection, Hainan University;1 Environment and Plant Protection Institute, CATAS2 Key Laboratory for Baleful Biology Detection and Monitor of Tropical Agriculture of Hainan Province;1 Environment and Plant Protection Institute, CATAS2 Key Laboratory for Baleful Biology Detection and Monitor of Tropical Agriculture of Hainan Province3 College of Environment and Plant Protection, Hainan University |
| |
| Abstract: | The phytoplasma of Hainan periwinkle yellows disease in spear and petiole was detected with DAPI fluorescence microscopy. The results showed that a large number of specific phytoplasma DNA fluorescent spots appeared in the sieve of phloem of diseased plants, but in the healthy plants, the specific phytoplasma fluorescence was not found in the sieve. In addition, the contents of phytoplasma in the stem and the petiole were compared. The results showed that the content of the stem is slightly higher than in the petioles. |
| |
| Keywords: | |
|
| 点击此处可从《热带作物学报》浏览原始摘要信息 |
| 点击此处可从《热带作物学报》下载免费的PDF全文 |
