| 猪繁殖与呼吸综合征病毒贵州流行株ORF5序列变异分析 |
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| 引用本文： | 方英,毛君婷,史开志,王开功,周碧君,汪德生,文明.猪繁殖与呼吸综合征病毒贵州流行株ORF5序列变异分析[J].贵州大学学报(农业与生物科学版),2009,28(1). |
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| 作者姓名： | 方英 毛君婷 史开志 王开功 周碧君 汪德生 文明 |
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| 作者单位： | 1. 贵州大学,动物科学学院,贵州,贵阳,550025
2. 贵州大学,动物疫病研究所,贵州,贵阳,550025 |
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| 基金项目： | 贵州省农业科技重大专项基金，贵阳市农业局科技计划项目，贵州省农业厅兽医科技计划项目 |
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| 摘 要： | 通过RT-PCR扩增9株贵州省PRRSV流行毒株的ORF5基因,并将其连接到pMD18-T载体进行克隆测序得到目的基因序列.参考国内外毒株,分析其变异特点.结果表明,9株PRRSV贵州流行株都属于美洲型毒株,其ORF5序列之间同源性为99.5%~100.0%.以GZCJ株为贵州省代表毒株,与美洲型参考毒株之间的同源性为89.7%~98.7%,与近年流行的毒株遗传距离较近,与早期流行毒株遗传距离较远,其发生了大量点突变.
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| 关 键 词： | 贵州省 猪繁殖与呼吸综合征病毒 ORF5序列 点突变 变异分析 |
Variation analysis of PRRSV-ORF5 sequence in Guizhou Province |
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| FANG Ying,MAO Jun-ting,SHI Kai-zhi,WANG Kai-gong,ZHOU Bi-junu,WANG De-sheng,WEN Ming.Variation analysis of PRRSV-ORF5 sequence in Guizhou Province[J].Journal of Mountain Agriculture & Biology,2009,28(1). |
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| Authors: | FANG Ying MAO Jun-ting SHI Kai-zhi WANG Kai-gong ZHOU Bi-junu WANG De-sheng WEN Ming |
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| Institution: | 1.College of Animal Science;Guizhou University;Guiyang Guizhou 550025;China;2.Institute of Animal Disease;China |
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| Abstract: | The ORF5 of 9 strains PRRSV in Guizhou Province were amplified by RT-PCR,and ligated into the pMD18-T vector and sequenced.Compared with others ORF5 sequences of PRRSV,its variation charaters Guizhou strains were analyzed.The result indicated that 9 strains of PRRSV in Guizhou Province belong to all North America type,whose sequence similarities are 99.5%-100% at nucleotide level.As a representative,GZCJ shares 89.7% to 98.7% homology with the reference strains at nucleotide level.It is closele related to t... |
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| Keywords: | PRRSV ORF5 sequence Point mutation Variation analysis |
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