牛趋化因子受体基因1第2外显子的单核苷酸多态性分析

采用DNA测序、巢氏PCR和CRS-PCR方法对中国荷斯坦牛、鲁西黄牛、渤海黑牛的趋化因子受体基因1(CXCR1)的单核苷酸多态性(SNPs)进行研究,在第2外显子上发现了4个SNPs,分别为291 bp(C/T)、333 bp(C/T)、337 bp(A/G)和365 bp(C/T),这4个位点在中国荷斯坦牛、鲁西黄牛、渤海黑牛群体中均达到中度多态(0.25PIC0.5),优势等位基因分别为C、C、A、C,等位基因频率分别为0.82/0.67/0.65、0.80/0.74/0.71、0.70/0.65/0.82、0.56/0.58/0.56。经χ2适合性检验,渤海黑牛所有位点全部达到Hardy-Weinberg平衡状态;荷斯坦牛在突变位点291 bp(C/T)、333 bp(C/T)和337 bp(A/G)达到Hardy-Weinberg平衡状态;鲁西黄牛仅在365 bp(C/T)位点达到Hardy-Weinberg平衡状态。

Single Nucleotide Polymorphisms within the Second Conspicuous Factor of CXCR1 Gene in Bos taurus

The single nucleotide polymorphisms (SNPs) within the chemokine receptor CXCR1 gene in Holstein cattle，Luxi Yellow cattle and Bohai Black cattle were investigated by DNA sequencing technology，nested-PCR and CRS-PCR methods.Sequencing results showed that four SNPs in exon 2 were identified，which they were 291 bp (C/T)，333 bp (C/T)，337 bp (A/G) and 365 bp (C/T).The value of polymorphism information content indicated(0.25＜PIC＜0.5) that all the four SNPs were moderate polymorphism in Holstein cattle,Luxi Yellow cattle and Bohai Black cattle.Furthermore，C, C， A and C predominan allele were identical at 291 bp (C/T)，333 bp (C/T)，337 bp (A/G) and 365 bp (C/T)，with the allelic frequencies 0.82/0.67/0.65，0.80/0.74/0.71，0.70/0.65/0.82，0.56/0.58/0.56 respectivly. Chi-square test indicated all the sites of Bohai Black cattle were in accordance with the Hardy-Weinberg equilibrium，while Holstein cattle meet Hardy-Weinberg equilibrium (P>0.05) at 291 bp (C/T)，333 bp (C/T) and 337 bp (A/G)，and the Luxi Yellow cattle meet Hardy-Weinberg equilibrium only at 365 bp (C/T).