分子氨对黄河鲤鱼血清抗氧化反应的影响

为了探讨养殖水域中分子氨对水生生物的毒性效应,在前期毒性试验的基础上设置3个试验组(分子氨质量浓度分别为0.071,0.143,0.284mg/L)、1个对照组对黄河鲤鱼(Cyprinus carpio haematopterus Temmink etSchlegel)进行毒性试验,在试验的7,14,28,35d取样检测,测定试验鱼血清超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活力和丙二醛(MDA)质量浓度.结果表明:随着分子氨质量浓度的增加,黄河鲤鱼血清中SOD酶活力呈先升后降的趋势,质量浓度为0.28mg/L时,SOD活力低于对照组,GSH-Px的活力和MDA质量浓度呈现上升的趋势,方差分析表明分子氨极显著地提高血清MDA质量浓度(p<0.01),二者存在剂量—效应关系;随着分子氨暴露时间的延长,SOD活力呈下降趋势,MDA质量浓度呈上升趋势,方差分析差异不显著(p>0.05).在试验质量浓度范围内分子氨可降低鲤鱼抗氧化能力,且MDA可作为一种有效的生物标志物评价分子氨的毒性.

Effect of Ammonia on Antioxidant Responses in Serum of Yellow River Carps

To study the toxic effects of ammonia on the aquatic species,juveniles of Yellow River carp were assigned to 4 groups with an ammonia nitrogen concentration of 0(control),0.071,0.143 and 0.284 mg/L respectively.Samples were taken on 7,14,28 and 35 days after the start of the treatments and SOD and GSH-Px activities and MDA content of the serum were determined.The results demonstrated that with increasing ammonia concentration,the activity of SOD in the serum first increased and then decreased,while the acti...