| AtICE1和AtCBF1~3基因表达载体的构建与转化烟草研究 |
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| 引用本文： | 王冬梅,王明,李翠萍,苏承刚,张兴国,姚永宏.AtICE1和AtCBF1~3基因表达载体的构建与转化烟草研究[J].西南农业学报,2011,24(3). |
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| 作者姓名： | 王冬梅 王明 李翠萍 苏承刚 张兴国 姚永宏 |
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| 作者单位： | 1. 南方山地园艺学教育部重点实验室,西南大学园艺园林学院，重庆，400715
2. 重庆市农业科学院，重庆，401329 |
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| 基金项目： | 重庆市科委重大攻关(CSTC2010AA1019); 农业部转基因重大专项(2009ZX08010-005B) |
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| 摘 要： | 植物抗寒属于复杂的数量性状。单一基因转化,植株抗寒性提高有限。本文利用拟南芥的AtCBF1~3和AtICE14个基因构建植物表达载体pVCT2276,通过农杆菌介导法转化烟草,获得35株抗性苗。经抗性筛选以及PCR鉴定,8株显示为阳性植株。将其中5株繁殖成株系,为后续的抗寒鉴定奠定基础。
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| 关 键 词： | 抗寒 AtCBF1~3基因 AtICE1基因 转基因烟草 |
Studies on Vector Construction and Tobacco Transformation with AtICE1 and AtCBF1-3 Cluster Genes |
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| WANG Dong-mei,WANG Ming,LI Cui-ping,SU Cheng-gang,ZHANG Xing-guo,YAO Yong-hong.Studies on Vector Construction and Tobacco Transformation with AtICE1 and AtCBF1-3 Cluster Genes[J].Southwest China Journal of Agricultural Sciences,2011,24(3). |
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| Authors: | WANG Dong-mei WANG Ming LI Cui-ping SU Cheng-gang ZHANG Xing-guo YAO Yong-hong |
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| Institution: | WANG Dong-mei1,WANG Ming1,LI Cui-ping1,SU Cheng-gang1,ZHANG Xing-guo1,YAO Yong-hong2(1.Kay Laboratory of Horticulture Science for Southern Mountainous Regions,Ministry of Education,Southwest University,Chongqing 400716,China,2.Chongqing Academy of Agicultural Sciences,Chongqing 401329,China) |
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| Abstract: | Cold-resistance in plants was determined by numerous genes.With transformation of the sole gene into plants,the Cold-resistance could be enhanced limitedly.Amplified from Arabidopsis thaliana genomic DNA and connected with Arabidopsis promoter 35S,AtCBF1-3 and AtICE1 genes were used to construct the cold-induced plant expressing vector pVCT2276.Thirty-five Kanamycin resistance plants were obtained by Agro-bacterium mediated genetic transformation.PCR results showed that 8 plants were positive.For later sout... |
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| Keywords: | Cold-resistance AtCBF1-3 AtICE1 Transgenic tobacco |
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