Extraction of chlorophyll a from biological soil crusts: A comparison of solvents for spectrophotometric determination |
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Authors: | Sarah C. Castle Conor D. Morrison Nichole N. Barger |
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Affiliation: | University of Colorado, Ecology and Evolutionary Biology, Boulder, CO, USA |
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Abstract: | We tested the efficacy of four different commonly used solvents (acetone, ethanol, dimethyl sulfoxide, methanol) for the extraction of chla from biological soil crusts of three different successional stages (dark, intermediate, and light). Our results indicate that a double extraction technique is necessary in order to achieve chla recovery in the range of 76-87 percent. For all crust types, ethanol and dimethyl sulfoxide extracted the greatest amount of chla using a two-extraction efficiency calculation. |
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Keywords: | Biological soil crust Chlorophyll a Dimethyl sulfoxide DMSO Ethanol Methanol Acetone |
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