| 顺式环氧琥珀酸水解酶酶活的快速测定方法 |
| |
| 引用本文： | 彭亮亮,王自强,王云山,张利平,苏志国. 顺式环氧琥珀酸水解酶酶活的快速测定方法[J]. 中国农业科技导报, 2010, 12(1): 135-138. DOI: 10.3969/j.issn.1008-0864.2010.01.23 |
| |
| 作者姓名： | 彭亮亮 王自强 王云山 张利平 苏志国 |
| |
| 作者单位： | (1.河北大学生命科学学院, 河北 保定 071002,2.中国科学院过程工程研究所生化工程国家重点实验室, 北京 100080) |
| |
| 摘 要： | 在对产顺式环氧琥珀酸水解酶（ESH）的细胞超声破碎的基础上，通过正交实验对细胞中ESH生物转化生产L(+)-酒石酸过程中的底物浓度、酶浓度、转化时间进行了分析，建立了一种快速测定ESH酶活的方法，即1 g湿细胞，30 mL 0.2 mol/L的顺式环氧琥珀酸二钠（ES），置于37℃保温转化10 min后，利用 HPLC测定酒石酸含量。此方法具有快速、准确和细胞用量少的特点，可用于菌种选育、发酵过程检测、发酵条件优化及工业生产。
|
| 关 键 词： | 顺式环氧琥珀酸水解酶 超声破碎 L-酒石酸 生物转化, |
| 收稿时间： | 2009-12-30 |
| 修稿时间： | 2010-01-11 |
Method for Rapid Measuring Cis-epoxysuccinate Hydrolase Activity |
| |
| PENG Liang-liang,WANG Zi-qiang,WANG Yun-shan,ZHANG Li-ping,SU Zhi-guo. Method for Rapid Measuring Cis-epoxysuccinate Hydrolase Activity[J]. Journal of Agricultural Science and Technology, 2010, 12(1): 135-138. DOI: 10.3969/j.issn.1008-0864.2010.01.23 |
| |
| Authors: | PENG Liang-liang WANG Zi-qiang WANG Yun-shan ZHANG Li-ping SU Zhi-guo |
| |
| Affiliation: | (1.College of Life Science, Heibei University, Hebei Baoding 071002|2. National Key Laboratory of ;Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, China) |
| |
| Abstract: | On the basis of cis-epoxysuccinate hydrolase(ESH) producing cell ultrasonication,orthogonal experiments were conducted to analyze the effect of substrate concentration,the enzyme concentration and the time of biotransformation on the activity of ESH in the process of L(+)-tartaric acid production with cis-epoxysuecinate as the substrate for fermentation.An extraordinarily faster method for measuring ESH activity was constructed,which was to put 1 g wet cells into 30 mL 0.2 mol/L ES aqueous solution under th... |
| |
| Keywords: | cis-epoxysuccinate hydrolase ultrasonication L(+)-tartaric acid biotransformation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《中国农业科技导报》浏览原始摘要信息 |
|
点击此处可从《中国农业科技导报》下载全文 |
