| 西瓜细菌性果斑病拮抗内生细菌的分离和筛选 |
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| 引用本文： | 蔡学清,鄢凤娇,林玉,胡方平. 西瓜细菌性果斑病拮抗内生细菌的分离和筛选[J]. 福建农林大学学报(自然科学版), 2009, 38(5) |
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| 作者姓名： | 蔡学清 鄢凤娇 林玉 胡方平 |
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| 作者单位： | 福建农林大学植物保护学院,福建,福州,350002 |
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| 基金项目： | 福建农林大学校青年基金资助项目 |
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| 摘 要： | 从葫芦科作物及空心菜的叶片、茎及花上共分离到95个菌株,采用抑菌圈法共筛选到对西瓜细菌性果斑病病原菌具有不同程度拮抗作用的菌株46株,占分离菌株总数的48.4%;从拮抗菌株中筛选出5株对西瓜细菌性果斑病菌具有较好拮抗效果的菌株,其对西瓜细菌性果斑病的防治效果为68.75%-93.24%.内生性测定结果表明这5株菌株均能在西瓜体内定殖,5株菌株经过革兰氏染色、鞭毛染色、芽孢染色和一些生理生化测定,初步被确定为芽孢杆菌属(Bacillus spp.).
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| 关 键 词： | 西瓜细菌性果斑病 拮抗 内生细菌 芽孢杆菌 |
Isolation and screening of the antagonistic and endophytic bacteria against Acidovorax avenae subsp.citrulli |
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| CAI Xue-qing,YAN Feng-jiao,LIN Yu,HU Fang-ping. Isolation and screening of the antagonistic and endophytic bacteria against Acidovorax avenae subsp.citrulli[J]. Journal of Fujian Agricultural and Forestry University, 2009, 38(5) |
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| Authors: | CAI Xue-qing YAN Feng-jiao LIN Yu HU Fang-ping |
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| Affiliation: | CAI Xue-qing,YAN Feng-jiao,LIN Yu,HU Fang-ping(College of Plant Protection,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China) |
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| Abstract: | Ninety five bacterial strains were isolated from water spinach(Ipomaea aquatica) and Cucurbitaceae plants,of which forty six endophytic bacterial strains could inhibit Acidovorax avenae subsp.citrulli,it accounted for 48.4%.Five strains were selected with the control effect of 68.75%-93.24%.The five strains could colonize in watermelon,and were identified as Bacillus spp.based on the results of Gram,flagella,spore stains and physiological and biochemical tests. |
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| Keywords: | Acidovorax avenae subsp.citrulli antagonism endophytic bacteria Bacillus spp. |
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