| 甘蔗叶片多糖的提取及体外抗氧化作用 |
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| 引用本文： | 桂意云,贤武,梁强,苏树权,杨荣仲.甘蔗叶片多糖的提取及体外抗氧化作用[J].西南农业学报,2012,25(4):1218-1221. |
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| 作者姓名： | 桂意云 贤武 梁强 苏树权 杨荣仲 |
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| 作者单位： | 广西甘蔗研究所/中国农业科学院甘蔗研究中心/国家糖料改良中心广西甘蔗品种改良分中心/广西甘蔗遗传改良重点实验室,广西南宁,530007 |
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| 基金项目： | 现代农业产业体系建设专项(Nycytx-024-01-04);广西农业科学院基本科研业务费项目(200802);广西甘蔗研究所重点实验室科研业务费项目(G2009016) |
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| 摘 要： | 应用正交试验优选甘蔗叶片多糖的热水浸提工艺,并进行叶片多糖体外抗氧化作用研究.结果显示最优条件为浸提温度80℃,料液比1:40,提取4次,每次浸提3h；在11月甘蔗生长后期,甘蔗叶片有较高的多糖含量；甘蔗叶片多糖具有较强清除·OH的能力,对·OH致DNA损伤具有良好的保护作用.
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| 关 键 词： | 甘蔗多糖 提取 羟基自由基 抗氧化作用 |
Extraction and Antioxidant Activity of Ploysaccharide from Sugarcane Leaves |
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| GUI Yi-yun
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XIAN Wu
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LIANG Qiang
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SU Shu-quan
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YANG Rong-zhong.Extraction and Antioxidant Activity of Ploysaccharide from Sugarcane Leaves[J].Southwest China Journal of Agricultural Sciences,2012,25(4):1218-1221. |
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| Authors: | GUI Yi-yun XIAN Wu LIANG Qiang SU Shu-quan YANG Rong-zhong |
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| Institution: | (Guangxi Sugarcane Research Institute /Sugarcane Research Center of Chinese Academy of Agricultural Sciences/ Guangxi Key Laboratory of Sugarcane Genetic Improvement /Guangxi Sugarcane Sub-center National Sugar Crops Improvement Center,Guangxi Nanning 530007,China) |
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| Abstract: | Orthogonal test was used to optimize the hot water technique of extracting ploysaccharide in sugarcane leaves,and anti-oxidation of ploysaccharide in vitro was studied.The optimum conditions were: extraction temperature of 80 ℃,the solid and solvent rations of 1∶ 40,extraction of four times,extraction time of 3h.At the later growth stage of sugarcane in November,the content of sugarcane polysaccharide was higher,and the scavenging effect on ·OH and inhibiting DNA damage generated by ·OH.were stronger. |
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| Keywords: | Sugarcane polysaccharide Extraction ·OH Anti-oxidation |
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