| 华东葡萄广西-2花药胚状体诱导与再生植株的研究 |
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| 引用本文： | 支玉玺,张剑侠,王跃进.华东葡萄广西-2花药胚状体诱导与再生植株的研究[J].果树学报,2010(1). |
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| 作者姓名： | 支玉玺 张剑侠 王跃进 |
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| 作者单位： | 农业部西北园艺植物种质利用重点开放实验室;陕西省农业分子生物学实验室;西北农林科技大学园艺学院; |
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| 基金项目： | 国家863计划项目(2007AA10Z182);;国家自然科学基金(30771493);;农业部948项目(2005-Z40);;现代农业产业技术体系专项资金资助 |
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| 摘 要： | 为了提供葡萄转抗病基因功能验证及遗传转化研究的再生技术体系,以中国野生华东葡萄(V.pseudoretic-ulata)感白粉病株系广西-2的花药为试材,进行了体细胞胚诱导与再生体系的建立,并探讨了次生胚的诱导与畸形萌发胚状体正常成苗的方法。结果表明,广西-2花药在B5+6-BA4.0mg·L-1+2,4-D0.7mg·L-1+CH1.0g·L-1+PVP1.0g·L-1+蔗糖30.0g·L-1培养基上继代3个月,可诱导胚性愈伤组织产生;B5+6-BA4.0mg·L-1+CH1.0g·L-1+PVP1.0g·L-1+蔗糖15.0g·L-1培养基有利于胚状体形成;胚状体在1/2B5+CH1.0g·L-1+PVP1.0g·L-1+蔗糖15.0g·L-1的液体培养基比相同成分固体培养基中早萌发14d左右;已萌发胚状体在WPM+IBA0.15mg·L-1+AC0.5g·L-1+蔗糖15.0g·L-1培养基上可以生根成苗;MS+6-BA1.5mg·L-1+IBA0.2mg·L-1+蔗糖30.0g·L-1培养基有利于畸形萌发胚状体正常成苗。发育早期胚状体在B5+6-BA1.0mg·L-1+2,4-D0.5mg·L-1+CH1.0g·L-1+PVP1.0g·L-1+蔗糖30.0g·L-1培养基上可形成次生胚。
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| 关 键 词： | 中国野生葡萄 华东葡萄 花药 胚状体诱导 再生植株 |
Studies on somatic embryogenesis and plant regeneration from anthers of Guangxi-2,a line of Chinese wild grape (Vitis pseudoreticulata) |
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| ZHI Yu-xi,ZHANG Jian-xia,WANG Yue-jin.Studies on somatic embryogenesis and plant regeneration from anthers of Guangxi-2,a line of Chinese wild grape (Vitis pseudoreticulata)[J].Journal of Fruit Science,2010(1). |
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| Authors: | ZHI Yu-xi ZHANG Jian-xia WANG Yue-jin |
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| Institution: | Northwest China's Key Laboratory of Horticultural Plant Germplasm Resources Utilization;Ministry of Agriculture;Shaanxi Key Laboratory of Agricultural Molecular Biology;College of Horticulture;Northwest A & F University;Yangling;Shaanxi 712100 China |
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| Abstract: | In order to offer a regeneration system for functional characterization of resistant genes in grapevine genetic trans-formation,anthers from powdery mildew-susceptible line Guangxi-2 of Chinese wild Vitis pseudoreticulata were used to induce somatic embryos and establish regeneration system. The induction of secondary embryos and the normal seedling from abnormal germinated somatic embryo were also explored in this study. The results indicated Guangxi-2 anthers could be induced and formed embryo callus on B... |
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| Keywords: | Chinese wild Vitis Vitis pseudoreticulata Anther Somatic embryo induction Regeneration plant |
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