| TP-M13-SSR技术及其在玉米遗传多样性研究中的应用 |
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| 引用本文： | 刘志斋, 王天宇, 黎裕.TP-M13-SSR技术及其在玉米遗传多样性研究中的应用[J].玉米科学,2007,15(6):010-015. |
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| 作者姓名： | 刘志斋 王天宇 黎裕 |
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| 作者单位： | 1. 中国农业科学院作物科学研究所,北京,100081;西南大学农学与生物技术学院,重庆,400716
2. 中国农业科学院作物科学研究所,北京,100081 |
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| 基金项目： | 科技部科技支撑计划;北京市自然科学基金重点项目;北京市农业育种基础研究创新平台项目 |
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| 摘 要： | 遗传多样性分析是种质资源研究的重要组成部分。由于研究群体通常都较大,简便可靠的高通量分析技术显得尤为重要。本文概述了在SSR扩增产物检测过程中的一种基于荧光测序技术的高通量低成本分析技术体系TP-M13-SSR(simple sequence repeat with tailed primer M13)的发展历程、反应原理和应用,并比较了它与普通银染、琼脂糖检测以及常规荧光检测体系在SSR扩增产物检测上的优劣,讨论了该技术在植物遗传多样性研究中的应用潜力。
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| 关 键 词： | 高通量 遗传多样性 |
| 文章编号： | 1005-0906（2007）06-0010-06 |
| 收稿时间： | 2007-08-12 |
| 修稿时间： | 2007-10-20 |
TP-M13-SSR Technique and Its Applications in the Analysis of Genetic Diversity in Maize |
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| LIU Zhi-zhai,WANG Tian-yu,LI Yu.TP-M13-SSR Technique and Its Applications in the Analysis of Genetic Diversity in Maize[J].Journal of Maize Sciences,2007,15(6):010-015. |
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| Authors: | LIU Zhi-zhai WANG Tian-yu LI Yu |
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| Abstract: | Genetic diversity analysis is one of important components in studies on germplasm resources. However, due to the huge size of samples tested in most studies, simple, reliable and high-throughput techniques are required. This paper briefly summarized an economical method of simple sequence repeat(SSR) detection in which fluorescent labeling of SSR-PCR products was used, with tailed primer M13 (TP-M13-SSR), including its development, reaction principles, and some key points. Its advantages and disadvantages were also compared with those of other detection methods, agarose electrophoresis and ordinary fluorescent labeling methods. In addition, the potential of application of the TP-M13-SSR technique in analysis of genetic diversity in maize was assessed. |
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| Keywords: | TP-M13-SSR |
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