磁化等离子体处理大豆种子的生物学效应

利用不同剂量的磁化等离子体处理晋豆20种子,分析被处理种子发芽率、幼苗生长、呼吸与能量的代谢以及同功酶谱的变化,为探讨磁化等离子体对种子的作用机理提供依据。结果表明,经不同剂量磁化等离子体处理后,种子的发芽率和幼苗的根长、干重没有显著变化;2.0～3.0A处理组苗高、鲜重比对照分别增加17.8%～26.9%和12.70%～17.08%;2.0A处理侧根数比对照显著增加,增幅为11.8%。当处理剂量为2.5A时,根系活力提高60.9%,叶片和根系的呼吸速率分别提高43.68%和38.92%,ATP含量分别提高37.79%和47.68%。磁化等离子体处理大豆种子后幼苗同工酶谱没有特异性条带的产生,但POD和酯酶同工酶的活性得到了普遍提高,其中2.5A处理后POD活性最高。综合分析指标表明2.5A的磁化等离子体处理对刺激种子活力、促进种子发芽和幼苗生长效果最佳。

BIOLOGICAL EFFECTS OF MAGNETIZED PLASMA ON SOYBEAN SEEDS

Soybean Jindou 20 seeds were treated by magnetized plasma with different dose, the seed germination, growth, respiratory, energy metabolism and isoenzyme patterns of seedlings were investigated. The results showed that no significant differences in seed germination rate, root length and dry weight of seedlings were detected under all treatments; however the seedling height and fresh weight increased by 17.8～26.9% and 12.70%～17.08% in the range of 2.0A～3.0A treatment intensity; lateral root number increased by 11.8% significantly (P<0.05) under 2.0A treatment intensity. When the treatment intensity was 2.5A, the root activity was improved by 60.9%; the respiratory rate of leaf and root were increased by 43.68% and 38.92%, and the ATP content of leaf and root were enhanced by 37.79% and 47.68%, respectively. Isoenzyme patterns of the peroxidase (POD) and esterase(EST) in leaf and root had almost no changes, but the POD and EST activity increased obviously after the plasma treatment. Comparative analysis indicated that 2.5A was the optimum treatment to stimulate seed vigor, promote seed germination and seedling growth.